During the past 15 years, the methylotrophic yeast Pichia pastoris has developed into a
highly successful system for the production of a variety of heterologous proteins. The …

The methylotrophic yeast Pichia pastoris is now one of the standard tools used in molecular
biology for the generation of recombinant protein. P. pastoris has demonstrated its most …

Angiogenesis is a complex process that involves endothelial cell proliferation, migration,
basement membrane degradation, and neovessel organization. Angiostatin, consisting of …

The mature form of the zymogen, human plasminogen (HPlg), contains 791 amino acids
present in a single polypeptide chain. The fibrinolytic enzyme, human plasmin (HPlm), is …

In the last few years the Pichia pastoris expression system has been gaining more and more
interest for the expression of recombinant proteins. Many groups have employed …

The X-ray crystal structure of the recombinant (r) kringle 5 domain of human plasminogen
(K5HPg) has been solved by molecular replacement methods using K1HPg as a model and …

Angiogenesis is a multi-step process that includes endothelial cell proliferation, migration,
basement membrane degradation, and new lumen organization. Angiostatin, an internal …

O‐linked saccharides were released from a major cell wall glycoprotein and from cellular
mannan–protein complexes obtained from Pichia pastoris cells. Analysis by a variety of …

The properties of recombinant staphylokinase (SakSTAR) expressed in Pichia pastoris cells
have been determined. The single consensus N-linked oligosaccharide linkage site in …

Although the roles of plasminogen and plasmin in mediating blood clot dissolution are well
known, the availability of mice deficient for components of the fibrinolytic system has allowed …