DNA metabarcoding for the characterization of terrestrial microbiota—pitfalls and solutions

D Francioli, G Lentendu, S Lewin, S Kolb - Microorganisms, 2021 - mdpi.com
Soil-borne microbes are major ecological players in terrestrial environments since they cycle
organic matter, channel nutrients across trophic levels and influence plant growth and …

Why Plasmodium vivax and Plasmodium falciparum are so different? A tale of two clades and their species diversities

AA Escalante, AS Cepeda, MA Pacheco - Malaria Journal, 2022 - Springer
The global malaria burden sometimes obscures that the genus Plasmodium comprises
diverse clades with lineages that independently gave origin to the extant human parasites …

Adapterama I: universal stubs and primers for 384 unique dual-indexed or 147,456 combinatorially-indexed Illumina libraries (iTru & iNext)

TC Glenn, RA Nilsen, TJ Kieran, JG Sanders… - PeerJ, 2019 - peerj.com
Massively parallel DNA sequencing offers many benefits, but major inhibitory cost factors
include:(1) start-up (ie, purchasing initial reagents and equipment);(2) buyin (ie, getting the …

Airborne environmental DNA metabarcoding for the monitoring of terrestrial insects—A proof of concept from the field

F Roger, HR Ghanavi, N Danielsson… - Environmental …, 2022 - Wiley Online Library
Biodiversity is in decline due to human‐induced pressures on ecosystems around the world.
To be able to counteract this alarming trend, it is paramount to closely monitor biodiversity at …

Adapterama I: universal stubs and primers for thousands of dual-indexed Illumina libraries (iTru & iNext)

TC Glenn, RA Nilsen, TJ Kieran, JW Finger Jr… - BioRxiv, 2016 - biorxiv.org
Next-generation DNA sequencing (NGS) offers many benefits, but major factors limiting NGS
include reducing the time and costs associated with: 1) start-up (ie, doing NGS for the first …

Enhanced Sequence-Activity Mapping and Evolution of Artificial Metalloenzymes by Active Learning

T Vornholt, M Mutný, GW Schmidt… - ACS Central …, 2024 - ACS Publications
Tailored enzymes are crucial for the transition to a sustainable bioeconomy. However,
enzyme engineering is laborious and failure-prone due to its reliance on serendipity. The …

The effect of ethanol concentration on the morphological and molecular preservation of insects for biodiversity studies

D Marquina, M Buczek, F Ronquist, P Łukasik - PeerJ, 2021 - peerj.com
Traditionally, insects collected for scientific purposes have been dried and pinned, or
preserved in 70% ethanol. Both methods preserve taxonomically informative exoskeletal …

Co‐occurrence of antibiotic, biocide, and heavy metal resistance genes in bacteria from metal and radionuclide contaminated soils at the Savannah River Site

JC Thomas IV, A Oladeinde, TJ Kieran… - Microbial …, 2020 - Wiley Online Library
Contaminants such as heavy metals may contribute to the dissemination of antimicrobial
resistance (AMR) by enriching resistance gene determinants via co‐selection mechanisms …

Horizontal Gene Transfer and Acquired Antibiotic Resistance in Salmonella enterica Serovar Heidelberg following In Vitro Incubation in Broiler Ceca

A Oladeinde, K Cook, SM Lakin, R Woyda… - Applied and …, 2019 - Am Soc Microbiol
The chicken gastrointestinal tract harbors microorganisms that play a role in the health and
disease status of the host. The cecum is the part of the gut that carries the highest microbial …

Phylogeography and cohesion species delimitation of California endemic trapdoor spiders within the Aptostichus icenoglei sibling species complex (Araneae …

LG Newton, J Starrett, EE Jochim… - Ecology and …, 2023 - Wiley Online Library
Species delimitation is an imperative first step toward understanding Earth's biodiversity, yet
what constitutes a species and the relative importance of the various processes by which …