[HTML][HTML] Bacillus subtilis as cell factory for pharmaceutical proteins: a biotechnological approach to optimize the host organism
L Westers, H Westers, WJ Quax - … et Biophysica Acta (BBA)-Molecular Cell …, 2004 - Elsevier
Bacillus subtilis is a rod-shaped, Gram-positive soil bacterium that secretes numerous
enzymes to degrade a variety of substrates, enabling the bacterium to survive in a …
enzymes to degrade a variety of substrates, enabling the bacterium to survive in a …
[PDF][PDF] Heterologous protein production and delivery systems for Lactococcus lactis
S Nouaille, LA Ribeiro, A Miyoshi, D Pontes… - Genet Mol …, 2003 - researchgate.net
Lactic acid bacteria (LAB), widely used in the food industry, are present in the intestine of
most animals, including humans. The potential use of these bacteria as live vehicles for the …
most animals, including humans. The potential use of these bacteria as live vehicles for the …
Functional Production and Characterization of a Fibrin-Specific Single-Chain Antibody Fragment from Bacillus subtilis: Effects of Molecular Chaperones and a Wall …
SC Wu, JC Yeung, Y Duan, R Ye… - Applied and …, 2002 - Am Soc Microbiol
To develop an ideal blood clot imaging and targeting agent, a single-chain antibody (SCA)
fragment based on a fibrin-specific monoclonal antibody, MH-1, was constructed and …
fragment based on a fibrin-specific monoclonal antibody, MH-1, was constructed and …
Recent Advances in Recombinant Protein Production by Bacillus subtilis
K Zhang, L Su, J Wu - Annual review of food science and …, 2020 - annualreviews.org
Bacillus subtilis has become a widely used microbial cell factory for the production of
recombinant proteins, especially those associated with foods and food processing. Recent …
recombinant proteins, especially those associated with foods and food processing. Recent …
Bottlenecks in the expression and secretion of heterologous proteins in Bacillus subtilis
W Li, X Zhou, P Lu - Research in microbiology, 2004 - Elsevier
Bacillus subtilis is an alternative host for expression and secretion of heterologous proteins.
However, low yields of protein production limit its use on a wide scale. The secretory …
However, low yields of protein production limit its use on a wide scale. The secretory …
Bacillus minimum genome factory: effective utilization of microbial genome information
K Ara, K Ozaki, K Nakamura, K Yamane… - Biotechnology and …, 2007 - Wiley Online Library
In 1997, the complete genomic DNA sequence of Bacillus subtilis (4.2 Mbp) was determined
and 4100 genes were identified [Kunst, Ogasawara, Moszer, Albertini, Alloni, Azevedo …
and 4100 genes were identified [Kunst, Ogasawara, Moszer, Albertini, Alloni, Azevedo …
Activation of subtilin precursors by Bacillus subtilis extracellular serine proteases subtilisin (AprE), WprA, and Vpr
C Corvey, T Stein, S Düsterhus, M Karas… - … and biophysical research …, 2003 - Elsevier
The maturation of the peptide antibiotic (lantibiotic) subtilin in Bacillus subtilis ATCC 6633
includes posttranslational modifications of the propeptide and proteolytic cleavage of the …
includes posttranslational modifications of the propeptide and proteolytic cleavage of the …
Proteomic analysis of Bacillus subtilis strains engineered for improved production of heterologous proteins
S Pohl, G Bhavsar, J Hulme, AE Bloor, G Misirli… - …, 2013 - Wiley Online Library
The use of bacterial systems for recombinant protein production has advantages of
simplicity, time and cost over competing systems. However, widely used bacterial …
simplicity, time and cost over competing systems. However, widely used bacterial …
Enhancement of extracellular expression of Bacillus naganoensis pullulanase from recombinant Bacillus subtilis: effects of promoter and host
W Song, Y Nie, XQ Mu, Y Xu - Protein Expression and Purification, 2016 - Elsevier
Pullulanase plays an important role in industrial applications of starch processing. However,
extracellular production of pullulanase from recombinant Bacillus subtilis is yet limited due to …
extracellular production of pullulanase from recombinant Bacillus subtilis is yet limited due to …
Stabilization of cell wall proteins in Bacillus subtilis: A proteomic approach
H Antelmann, H Yamamoto, J Sekiguchi… - Proteomics, 2002 - Wiley Online Library
Even though cell wall proteins of Bacillus subtilis are characterized by specific cell wall
retention signals, some of these are also components of the extracellular proteome. In …
retention signals, some of these are also components of the extracellular proteome. In …