The three RAS genes—HRAS, NRAS and KRAS—are collectively mutated in one-third of
human cancers, where they act as prototypic oncogenes. Interestingly, there are rather …

Abstract Error-corrected Next Generation Sequencing (ecNGS) is rapidly emerging as a
valuable, highly sensitive and accurate method for detecting and characterizing mutations in …

The ability to accurately measure mutations is critical for basic research and identifying
potential drug and chemical carcinogens. Current methods for in vivo quantification of …

Mutations have a profound effect on human health, particularly through an increased risk of
carcinogenesis and genetic disease. The strong correlation between mutagenesis and …

Exposure to environmental mutagens increases the risk of cancer and genetic disorders. We
used Duplex Sequencing (DS), a high-accuracy error-corrected sequencing technology, to …

Rats are a standard experimental animal for cancer bioassay and toxicological research for
chemicals. Although the genetic analyses were behind mice, rats have been more frequently …

It is difficult to identify mutagen-induced genome-wide somatic mutations using next
generation sequencing; hence, mutagenic features of each mutagen and their roles in …

Transgenic rodent (TGR) models use bacterial reporter genes to quantify in vivo
mutagenesis. Pairing TGR assays with next-generation sequencing (NGS) enables …

Understanding the effects of environmental exposures on germline mutation rates has been
a decades-long pursuit in genetics. We used next-generation sequencing and comparative …

Motivation Benzo [a] pyrene, a notorious DNA-damaging carcinogen, belongs to the family
of polycyclic aromatic hydrocarbons commonly found in tobacco smoke. Surprisingly …