HUH endonucleases are numerous and widespread in all three domains of life. The major
function of these enzymes is processing a range of mobile genetic elements by catalysing …

Staphylococcus aureus is a common cause of hospital, community and livestock-associated
infections and is increasingly resistant to multiple antimicrobials. A significant proportion of …

Integrating conjugative elements (ICEs) are self-transmissible, mobile elements that are
widespread among bacteria. Following their excision from the chromosome, ICEs transfer by …

Background Bacterial plasmids have a major impact on metabolic function and adaptation of
their hosts. An indigenous plasmid was identified in a Chinese isolate (GX01) of the invasive …

Type IV secretion system (T4SS) substrates are recruited through a translocation signal that
is poorly defined for conjugative relaxases. The relaxase TrwC of plasmid R388 is …

Bacterial conjugation is a mechanism of horizontal DNA transfer. The relaxase TrwC of the
conjugative plasmid R388 cleaves one strand of the transferred DNA at the oriT gene …

Background Bacterial conjugation is a mechanism for horizontal DNA transfer between
bacteria which requires cell to cell contact, usually mediated by self-transmissible plasmids …

TrwC is a bacterial protein involved in conjugative transfer of plasmid R388. It is transferred
together with the DNA strand into the recipient bacterial cell, where it can integrate the …

During conjugation, a single strand of DNA is cleaved at the origin of transfer (oriT) by the
plasmid-encoded relaxase. This strand is then unwound from its complement and …

The widely used pSU8 family of cloning vectors is based on a p15A replicon and a
chloramphenicol acetyltransferase (cat) gene conferring chloramphenicol resistance. We …