Microbial extracellular polymeric substances in the environment, technology and medicine

HC Flemming, ED van Hullebusch, BJ Little… - Nature Reviews …, 2024 - nature.com
Microbial biofilms exhibit a self-produced matrix of extracellular polymeric substances (EPS),
including polysaccharides, proteins, extracellular DNA and lipids. EPS promote interactions …

Recent trends in glycoproteomics by characterization of intact glycopeptides

S Piovesana, C Cavaliere, A Cerrato, A Laganà… - Analytical and …, 2023 - Springer
This trends article provides an overview of the state of the art in the analysis of intact
glycopeptides by proteomics technologies based on LC–MS analysis. A brief description of …

Surface-layer protein is a public-good matrix exopolymer for microbial community organisation in environmental anammox biofilms

L Li Wong, Y Lu, JCS Ho, S Mugunthan, Y Law… - The ISME …, 2023 - academic.oup.com
Extracellular polymeric substances (EPS) are core biofilm components, yet how they
mediate interactions within and contribute to the structuring of biofilms is largely unknown …

Physiological and stoichiometric characterization of ethanol-based chain elongation in the absence of short-chain carboxylic acids

MT Allaart, BB Fox, IHMS Nettersheim, M Pabst… - Scientific Reports, 2023 - nature.com
Hexanoate is a valuable chemical that can be produced by microorganisms that convert
short-chain-to medium-chain carboxylic acids through a process called chain elongation …

Full-length single-molecule protein fingerprinting

M Filius, R van Wee, C de Lannoy… - Nature …, 2024 - nature.com
Proteins are the primary functional actors of the cell. While proteoform diversity is known to
be highly biologically relevant, current protein analysis methods are of limited use for …

[HTML][HTML] Metaproteomics, metagenomics and 16S rRNA sequencing provide different perspectives on the aerobic granular sludge microbiome

HBC Kleikamp, D Grouzdev, P Schaasberg… - Water research, 2023 - Elsevier
The tremendous progress in sequencing technologies has made DNA sequencing routine
for microbiome studies. Additionally, advances in mass spectrometric techniques have …

Production of medium‐chain‐length PHA in octanoate‐fed enrichments dominated by Sphaerotilus sp.

CM Vermeer, L Depaz, E van den Berg… - Biotechnology and …, 2023 - Wiley Online Library
Medium‐chain‐length polyhydroxyalkanoate (mcl‐PHA) production by using microbial
enrichments is a promising but largely unexplored approach to obtain elastomeric …

[HTML][HTML] A systematic evaluation of yeast sample preparation protocols for spectral identifications, proteome coverage and post-isolation modifications

M den Ridder, E Knibbe, W van den Brandeler… - Journal of …, 2022 - Elsevier
The importance of obtaining comprehensive and accurate information from cellular
proteomics experiments asks for a systematic investigation of sample preparation protocols …

Differentiation of the Anammox core microbiome: Unraveling the evolutionary impetus of scalable gene flow

Q Zhang, XM Ji, X Wang, W Wang, X Xu, Q Zhang… - Water Research, 2025 - Elsevier
Anaerobic ammonium oxidation bacteria (AAOB), distinguished by their unique autotrophic
nitrogen metabolism, hold pivotal positions in the global nitrogen cycle and environmental …

Osmoregulation in freshwater anaerobic methane-oxidizing archaea under salt stress

MJ Echeveste Medrano, AO Leu, M Pabst… - The ISME …, 2024 - academic.oup.com
Climate change–driven sea level rise threatens freshwater ecosystems and elicits salinity
stress in microbiomes. Methane emissions in these systems are largely mitigated by …