Proteins are the main targets of most drugs; however, system-wide methods to monitor
protein activity and function are still underused in drug discovery. Novel biochemical …

Many biological processes are executed and regulated through the molecular interactions of
proteins and nucleic acids. Proximity labeling (PL) is a technology for tagging the …

This protocol describes the use of TurboID and split-TurboID in proximity labeling
applications for mapping protein–protein interactions and subcellular proteomes in live …

The renin-angiotensin-aldosterone system plays crucial roles in cardiovascular physiology
and pathophysiology. However, many of the signaling mechanisms have been unclear. The …

Protein interaction networks and protein compartmentalization underlie all signaling and
regulatory processes in cells. Enzyme-catalyzed proximity labeling (PL) has emerged as a …

G protein-coupled receptors (GPCRs) are the largest group of cell surface receptors in
humans that signal in response to diverse inputs and regulate a plethora of cellular …

We introduce APEX-seq, a method for RNA sequencing based on direct proximity labeling of
RNA using the peroxidase enzyme APEX2. APEX-seq in nine distinct subcellular locales …

Protein subcellular localization is tightly controlled and intimately linked to protein function in
health and disease. Capturing the spatial proteome—that is, the localizations of proteins and …

G protein-coupled receptors (GPCRs), which are modulated by a variety of endogenous and
synthetic ligands, represent the largest family of druggable targets in the human genome …

The ability to map trafficking for thousands of endogenous proteins at once in living cells
would reveal biology currently invisible to both microscopy and mass spectrometry. Here, we …