A brief guideline for studies of phase-separated biomolecular condensates

Y Gao, X Li, P Li, Y Lin - Nature Chemical Biology, 2022 - nature.com
Cells are exquisitely compartmentalized to achieve precise spatiotemporal regulation of
myriad processes and pathways. Phase separation offers one way to achieve territorial …

[HTML][HTML] Molecular mechanisms of stress granule assembly and disassembly

S Hofmann, N Kedersha, P Anderson… - Biochimica et Biophysica …, 2021 - Elsevier
Stress granules (SGs) are membrane-less ribonucleoprotein (RNP)-based cellular
compartments that form in the cytoplasm of a cell upon exposure to various environmental …

Competing protein-RNA interaction networks control multiphase intracellular organization

DW Sanders, N Kedersha, DSW Lee, AR Strom… - Cell, 2020 - cell.com
Liquid-liquid phase separation (LLPS) mediates formation of membraneless condensates
such as those associated with RNA processing, but the rules that dictate their assembly …

[HTML][HTML] Single-molecule imaging reveals translation of mRNAs localized to stress granules

D Mateju, B Eichenberger, F Voigt, J Eglinger, G Roth… - Cell, 2020 - cell.com
Cellular stress leads to reprogramming of mRNA translation and formation of stress granules
(SGs), membraneless organelles consisting of mRNA and RNA-binding proteins. Although …

A new phase of networking: the molecular composition and regulatory dynamics of mammalian stress granules

SR Millar, JQ Huang, KJ Schreiber, YC Tsai… - Chemical …, 2023 - ACS Publications
Stress granules (SGs) are cytosolic biomolecular condensates that form in response to
cellular stress. Weak, multivalent interactions between their protein and RNA constituents …

Higher-order organization of biomolecular condensates

CM Fare, A Villani, LE Drake, J Shorter - Open biology, 2021 - royalsocietypublishing.org
A guiding principle of biology is that biochemical reactions must be organized in space and
time. One way this spatio-temporal organization is achieved is through liquid–liquid phase …

Spatiotemporal proteomic analysis of stress granule disassembly using APEX reveals regulation by SUMOylation and links to ALS pathogenesis

H Marmor-Kollet, A Siany, N Kedersha, N Knafo… - Molecular cell, 2020 - cell.com
Stress granules (SGs) are cytoplasmic assemblies of proteins and non-translating mRNAs.
Whereas much has been learned about SG formation, a major gap remains in …

Stress-related biomolecular condensates in plants

J Solis-Miranda, M Chodasiewicz, A Skirycz… - The Plant …, 2023 - academic.oup.com
Biomolecular condensates are membraneless organelle-like structures that can concentrate
molecules and often form through liquid-liquid phase separation. Biomolecular condensate …

Mechanisms and regulation of RNA condensation in RNP granule formation

D Tauber, G Tauber, R Parker - Trends in biochemical sciences, 2020 - cell.com
Ribonucleoprotein (RNP) granules are RNA–protein assemblies that are involved in
multiple aspects of RNA metabolism and are linked to memory, development, and disease …

Inhibition of anti-viral stress granule formation by coronavirus endoribonuclease nsp15 ensures efficient virus replication

B Gao, X Gong, S Fang, W Weng, H Wang… - PLoS …, 2021 - journals.plos.org
Cytoplasmic stress granules (SGs) are generally triggered by stress-induced translation
arrest for storing mRNAs. Recently, it has been shown that SGs exert anti-viral functions due …