Abstract Functional Protein Engineering became the hallmark in biomolecule manipulation
in the new millennium, building on and surpassing the underlying structural DNA …

RNA structure is crucial to a wide range of cellular processes. The intimate relationship
between macromolecular structure and function necessitates the determination of high …

Ribozymes that react with small-molecule probes have important applications in
transcriptomics and chemical biology, such as RNA labeling and imaging. Understanding …

Pepper is a fluorogenic RNA aptamer tag that binds to a variety of benzylidene-cyanophenyl
(HBC) derivatives with tight affinity and activates their fluorescence. To investigate how …

Picornaviral IRES elements are essential for initiating the cap-independent viral translation.
However, three-dimensional structures of these elements remain elusive. Here, we report a …

Antibody fragments such as Fabs possess properties that can enhance protein and RNA
crystallization and therefore can facilitate macromolecular structure determination. In …

Structured RNA elements within the internal ribosome entry site (IRES) of hepatitis C virus
(HCV) genome hijack host cell machinery for translation initiation through a cap …

Recent advances in transcriptome sequencing and analysis have revealed the complexity of
the human genome. The majority (≈ 98%) of cellular transcripts is not translated into …

The interaction of Tudor domain-containing proteins (TDRDs) with P-element-induced
wimpy testis (PIWI) proteins plays critical roles in transposon silencing and …

RNA plays a central role in all organisms and can fold into complex structures to orchestrate
function. Visualization of such structures often requires crystallization, which can be a …