Design of artificial enzymes bearing several active centers: New trends, opportunities and problems

D Carballares, R Morellon-Sterling… - International Journal of …, 2022 - mdpi.com
Harnessing enzymes which possess several catalytic activities is a topic where intense
research has been carried out, mainly coupled with the development of cascade reactions …

Self-assembly systems to troubleshoot metabolic engineering challenges

Z Chen, T Wu, S Yu, M Li, X Fan, YX Huo - Trends in Biotechnology, 2024 - cell.com
Enzyme self-assembly is a technology in which enzyme units can aggregate into ordered
macromolecules, assisted by scaffolds. In metabolic engineering, self-assembly strategies …

The Effects of Buffer Nature on Immobilized Lipase Stability Depend on Enzyme Support Loading

P Abellanas-Perez, D Carballares, J Rocha-Martin… - Catalysts, 2024 - mdpi.com
The lipases from Thermomyces lanuginosus (TLL) and Candida antarctica (B)(CALB) were
immobilized on octyl-agarose beads at 1 mg/g (a loading under the capacity of the support) …

Multienzymatic catalysis and Enzyme Co-Immobilization

R Fernandez-Lafuente - Catalysts, 2023 - mdpi.com
The evolution of biocatalysis has undergone an unprecedented boost in response to the
human demand for sustainable chemistry, which should enable researchers to make the …

Combining Flexible Region Design and Automatic Design to Enhance the Thermal Stability and Catalytic Efficiency of Leucine Dehydrogenase

X Zhang, X Zhang, H Shi, H Zhang… - Journal of Agricultural …, 2024 - ACS Publications
Leucine dehydrogenase (LeuDH, EC 1.4. 1.9) can reversibly catalyze the oxidative
deamination of l-leucine and some other specific α-amino acids to form the corresponding α …

Engineering artificial fusion naringinase for enhancing naringenin biosynthesis

CM Luo, WT Zhang, SY Xie, XY Zhuang… - Biochemical …, 2024 - Elsevier
Naringinase (NGase) is a multi-compound enzyme with the subunits of α-L-rhamnosidase
(Rha) and β-D-glucosidase (BGL). The biotransformation of naringin into the more valuable …

Construction novel yeast surface-displaying glucose-leucine dehydrogenase fusions for cofactor recycling and chiral amino acid biosynthesis

J Deng, Z Yu, X Huang, W Xiong, K Jing - Biochemical Engineering Journal, 2024 - Elsevier
Whole-cell biosynthesis of chiral amino acids (AAs) is of significant potential application.
However, the difficulty of reactant translocation cell membranes limits their industrial …

Enhancing the thermostability of the leucine dehydrogenase from Planifilum fimeticola through rational design of the flexible region

XC Zhang, HL Shi, HM Zhang, JH Zhang, YC Kan… - Molecular …, 2024 - Elsevier
LeuDH catalyzes the reversible deamination of L-leucine and certain branched-chain
aliphatic amino acids, resulting in the corresponding α-keto acids. This process has received …

酶促合成手性氨基酸的研究进展

王子渊, 杨立荣, 吴坚平, 郑文隆 - 合成生物学, 2024 - synbioj.cip.com.cn
手性氨基酸是一类重要的高价值化学品, 广泛应用于食品, 医药, 化工, 农药等多个领域.
手性氨基酸常用的制备方法可以分为四类, 包括化学合成, 蛋白质水解, 发酵和酶促合成. 其中 …

A review on enzyme-catalyzed synthesis of chiral amino acids.

Z WANG, L YANG, J WU, W ZHENG - Synthetic Biology Journal, 2024 - synbioj.cip.com.cn
Chiral amino acids represent a crucial class of chiral building blocks with significant value in
food, medicine, chemical industry, and agriculture. The market scale of pharmaceuticals …