In vitro models of the blood–brain barrier: an overview of commonly used brain endothelial cell culture models and guidelines for their use

HC Helms, NJ Abbott, M Burek… - Journal of Cerebral …, 2016 - journals.sagepub.com
The endothelial cells lining the brain capillaries separate the blood from the brain
parenchyma. The endothelial monolayer of the brain capillaries serves both as a crucial …

Tight junctions of the blood–brain barrier: development, composition and regulation

H Wolburg, A Lippoldt - Vascular pharmacology, 2002 - Elsevier
1. The blood–brain barrier is essential for the maintenance and regulation of the neural
microenvironment. The main characteristic features of blood–brain barrier endothelial cells …

Microbiome–host systems interactions: protective effects of propionate upon the blood–brain barrier

L Hoyles, T Snelling, UK Umlai, JK Nicholson… - Microbiome, 2018 - Springer
Background Gut microbiota composition and function are symbiotically linked with host
health and altered in metabolic, inflammatory and neurodegenerative disorders. Three …

A perfused human blood–brain barrier on-a-chip for high-throughput assessment of barrier function and antibody transport

NR Wevers, DG Kasi, T Gray, KJ Wilschut… - Fluids and Barriers of …, 2018 - Springer
Background Receptor-mediated transcytosis is one of the major routes for drug delivery of
large molecules into the brain. The aim of this study was to develop a novel model of the …

A branched-chain amino acid metabolite drives vascular fatty acid transport and causes insulin resistance

C Jang, SF Oh, S Wada, GC Rowe, L Liu, MC Chan… - Nature medicine, 2016 - nature.com
Epidemiological and experimental data implicate branched-chain amino acids (BCAAs) in
the development of insulin resistance, but the mechanisms that underlie this link remain …

LRG1 promotes angiogenesis by modulating endothelial TGF-β signalling

X Wang, S Abraham, JAG McKenzie, N Jeffs, M Swire… - Nature, 2013 - nature.com
Aberrant neovascularization contributes to diseases such as cancer, blindness and
atherosclerosis, and is the consequence of inappropriate angiogenic signalling. Although …

Blood‐brain barrier‐specific properties of a human adult brain endothelial cell line

BB Weksler, EA Subileau, N Perriere… - The FASEB …, 2005 - Wiley Online Library
Establishment of a human model of the blood‐brain barrier has proven to be a difficult goal.
To accomplish this, normal human brain endothelial cells were transduced by lentiviral …

Reversal of endothelial dysfunction reduces white matter vulnerability in cerebral small vessel disease in rats

RM Rajani, S Quick, SR Ruigrok, D Graham… - Science translational …, 2018 - science.org
Dementia is a major social and economic problem for our aging population. One of the most
common of dementia in the elderly is cerebral small vessel disease (SVD). Magnetic …

Design and validation of a human brain endothelial microvessel-on-a-chip open microfluidic model enabling advanced optical imaging

MM Salman, G Marsh, I Kusters, M Delincé… - … in Bioengineering and …, 2020 - frontiersin.org
We describe here the design and implementation of an in vitro microvascular open model
system using human brain microvascular endothelial cells. The design has several …

Permeability studies on in vitro blood–brain barrier models: physiology, pathology, and pharmacology

MA Deli, CS Ábrahám, Y Kataoka, M Niwa - Cellular and molecular …, 2005 - Springer
The specifically regulated restrictive permeability barrier to cells and molecules is the most
important feature of the blood–brain barrier (BBB). The aim of this review was to summarize …