Computational coherent Raman scattering imaging: breaking physical barriers by fusion of advanced instrumentation and data science

H Lin, JX Cheng - eLight, 2023 - Springer
Coherent Raman scattering (CRS) microscopy is a chemical imaging modality that provides
contrast based on intrinsic biomolecular vibrations. To date, endeavors on instrumentation …

Rapid single-particle chemical imaging of nanoplastics by SRS microscopy

N Qian, X Gao, X Lang, H Deng… - Proceedings of the …, 2024 - National Acad Sciences
Plastics are now omnipresent in our daily lives. The existence of microplastics (1 µm to 5 mm
in length) and possibly even nanoplastics (< 1 μm) has recently raised health concerns. In …

Quantitative stimulated Raman scattering microscopy: promises and pitfalls

B Manifold, D Fu - Annual Review of Analytical Chemistry, 2022 - annualreviews.org
Since its first demonstration, stimulated Raman scattering (SRS) microscopy has become a
powerful chemical imaging tool that shows promise in numerous biological and biomedical …

Instant diagnosis of gastroscopic biopsy via deep-learned single-shot femtosecond stimulated Raman histology

Z Liu, W Su, J Ao, M Wang, Q Jiang, J He, H Gao… - Nature …, 2022 - nature.com
Gastroscopic biopsy provides the only effective method for gastric cancer diagnosis, but the
gold standard histopathology is time-consuming and incompatible with gastroscopy …

Ovarian cancer cell fate regulation by the dynamics between saturated and unsaturated fatty acids

G Zhao, Y Tan, H Cardenas… - Proceedings of the …, 2022 - National Acad Sciences
Fatty acids are an important source of energy and a key component of phospholipids in
membranes and organelles. Saturated fatty acids (SFAs) are converted into unsaturated fatty …

Profiling single cancer cell metabolism via high-content SRS imaging with chemical sparsity

Y Tan, H Lin, JX Cheng - Science Advances, 2023 - science.org
Metabolic reprogramming in a subpopulation of cancer cells is a hallmark of tumor
chemoresistance. However, single-cell metabolic profiling is difficult because of the lack of a …

Single-cell mapping of lipid metabolites using an infrared probe in human-derived model systems

Y Bai, CM Camargo, SMK Glasauer, R Gifford… - Nature …, 2024 - nature.com
Understanding metabolic heterogeneity is the key to uncovering the underlying mechanisms
of metabolic-related diseases. Current metabolic imaging studies suffer from limitations …

In situ and real-time mold flux analysis using a high-temperature fiber-optic Raman sensor for steel manufacturing applications

B Zhang, H Tekle, RJ O'Malley, T Sander… - Journal of Lightwave …, 2023 - opg.optica.org
Continuous casting in steel production uses specially developed oxyfluoride glasses (mold
fluxes) to lubricate a mold and control the solidification of the steel in the mold. The …

Deep learning autofluorescence-harmonic microscopy

B Shen, S Liu, Y Li, Y Pan, Y Lu, R Hu, J Qu… - Light: Science & …, 2022 - nature.com
Laser scanning microscopy has inherent tradeoffs between imaging speed, field of view
(FOV), and spatial resolution due to the limitations of sophisticated mechanical and optical …

Multi-molecular hyperspectral PRM-SRS microscopy

W Zhang, Y Li, AA Fung, Z Li, H Jang, H Zha… - Nature …, 2024 - nature.com
Lipids play crucial roles in many biological processes. Mapping spatial distributions and
examining the metabolic dynamics of different lipid subtypes in cells and tissues are critical …