Review of Levan polysaccharide: From a century of past experiences to future prospects

ET Öner, L Hernández, J Combie - Biotechnology advances, 2016 - Elsevier
Levan is a fascinating β-(2, 6)-linked fructose polymer with an unusual combination of
properties characterized in this review. In nature, levan is synthesized from sucrose by a …

Recent advances in levansucrase and inulosucrase: evolution, characteristics, and application

W Xu, D Ni, W Zhang, C Guang, T Zhang… - Critical reviews in food …, 2019 - Taylor & Francis
Levan and inulin are two types of fructan. Levan is composed of β-(2, 6) fructosyl linkage
and inulin is composed of β-(2, 1) linkage. Both levan and inulin have been accepted and …

[HTML][HTML] Candidate acetic acid bacteria strains for levan production

K Anguluri, S La China, M Brugnoli, L De Vero… - Polymers, 2022 - mdpi.com
In this study, twelve strains of acetic acid bacteria (AAB) belonging to five different genera
were tested for their ability to produce levan, at 70 and 250 g/L of sucrose concentration …

Biosynthesis of levan from sucrose using a thermostable levansucrase from Lactobacillus reuteri LTH5448

D Ni, W Xu, Y Bai, W Zhang, T Zhang, W Mu - International Journal of …, 2018 - Elsevier
Levan, a versatile fructan, possesses promising prospects for application in the food and
pharmaceutical industries and in many other fields due to the superior physicochemical …

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2013–2014

DJ Harvey - Mass Spectrometry Reviews, 2018 - Wiley Online Library
This review is the eighth update of the original article published in 1999 on the application of
Matrix‐assisted laser desorption/ionization mass spectrometry (MALDI) mass spectrometry …

Successful Manipulation of the Product Spectrum of the Erwinia amylovora Levansucrase by Modifying the Residues around loop1, Loop 3, and Loop 4

X Zhang, W Xu, D Ni, W Zhang… - Journal of Agricultural …, 2022 - ACS Publications
Levansucrase (LS, EC 2.4. 1.10) catalyzes the synthesis of levan by successively
transferring the fructosyl moiety from sucrose to an elongated fructan chain. Although the …

Modulation of fructooligosaccharide chain length and insight into the product binding motif of Lactobacillus reuteri 121 inulosucrase

T Charoenwongpaiboon, T Sitthiyotha… - Carbohydrate …, 2019 - Elsevier
Abstract Inulosucrase (EC 2.4. 1.9) is a bacterial fructosyltransferase that synthesizes inulin-
type fructooligosaccharide, using sucrose as a substrate. We modulated the size of …

Recent novel applications of levansucrases

W Li, S Yu, T Zhang, B Jiang, W Mu - Applied microbiology and …, 2015 - Springer
Levansucrase catalyzes three distinct reactions depending on the fructosyl acceptor
molecule, including polymerization, transfructosylation, and hydrolysis. As a key biocatalyst …

[HTML][HTML] Degradation of Fructans and Production of Propionic Acid by Bacteroides thetaiotaomicron are Enhanced by the Shortage of Amino Acids

S Adamberg, K Tomson, H Vija, M Puurand… - Frontiers in …, 2014 - frontiersin.org
Bacteroides thetaiotaomicron is commonly found in the human colon and stabilizes its
ecosystem by catabolism of various polysaccharides. A model of cross-talk between the …

The crystal structure of Erwinia amylovora levansucrase provides a snapshot of the products of sucrose hydrolysis trapped into the active site

J Wuerges, L Caputi, M Cianci, S Boivin… - Journal of Structural …, 2015 - Elsevier
Levansucrases are members of the glycoside hydrolase family and catalyse both the
hydrolysis of the substrate sucrose and the transfer of fructosyl units to acceptor molecules …