Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …

Expansion microscopy (ExM) is a newly developed super-resolution technique, allowing
visualization of biological targets at nanoscale resolution on conventional fluorescence …

Decoding cellular processes requires visualization of the spatial distribution and dynamic
interactions of biomolecules. It is therefore not surprising that innovations in imaging …

Resolving the distribution of specific proteins at the nanoscale in the ultrastructural context of
the cell is a major challenge in fluorescence microscopy. We report the discovery of a new …

With few-nanometer resolution recently achieved by a new generation of fluorescence
nanoscopes (MINFLUX and MINSTED), the size of the tags used to label proteins will …

Eukaryotic cells use clathrin-mediated endocytosis to take up a large range of extracellular
cargo. During endocytosis, a clathrin coat forms on the plasma membrane, but it remains …

Despite the enormous potential of nanomedicines to shape the future of medicine, their
clinical translation remains suboptimal. Translational challenges are present in every step of …

Optical investigations of nanometer distances between proteins, their subunits, or other
biomolecules have been the exclusive prerogative of Förster resonance energy transfer …

ABSTRACT A milestone in the field of recombinant binding molecules was achieved 30
years ago with the discovery of single-domain antibodies from which antigen-binding …

Over the past two decades, super-resolution microscopy has seen a tremendous
development in speed and resolution, but for most of its methods, there exists a remarkable …