Emerging evidence suggests that liquid–liquid phase separation (LLPS) represents a vital
and ubiquitous phenomenon underlying the formation of membraneless organelles in …

Cells compartmentalize their intracellular environment to orchestrate countless
simultaneous biochemical processes. Many intracellular tasks rely on membrane-less …

The cilium is an antenna-like organelle that performs numerous cellular functions, including
motility, sensing, and signaling. The base of the cilium contains a selective barrier that …

Automated tomographic reconstruction is now possible in the IMOD software package,
including the merging of tomograms taken around two orthogonal axes. Several …

Macromolecular crowding has a profound impact on reaction rates and the physical
properties of the cell interior, but the mechanisms that regulate crowding are poorly …

Cryogenic-electron tomography enables the visualization of cellular environments in
extreme detail, however, tools to analyze the full amount of information contained within …

Summary Approximately 30%–40% of global CO 2 fixation occurs inside a non-membrane-
bound organelle called the pyrenoid, which is found within the chloroplasts of most …

Nuclear magnetic resonance (NMR) spectroscopy is a well-established method for
analyzing protein structure, interaction, and dynamics at atomic resolution and in various …

Cryo-electron microscopy (cryo-EM) has emerged as a powerful structure determination
technique. Its most prolific branch is single particle analysis (SPA), a method being used in a …

Despite its short history, diffraction-unlimited fluorescence microscopy techniques have
already made a substantial imprint in the biological sciences. In this review, we describe …