This paper aims to discuss various aspects of the use of reference genes in qPCR technique
used in the thousands of present studies. Most frequently, these are housekeeping genes …

The root bacterial microbiome is important for the general health of the plant. Additionally, it
can enhance tolerance to abiotic stresses, exemplified by plant species found in extreme …

Many reference genes are used by different laboratories for gene expression analyses to
indicate the relative amount of input RNA/DNA in the experiment. These reference genes …

Background The development of CRISPR/Cas9 technology has facilitated targeted
mutagenesis in an efficient and precise way. Previously, RNAi silencing of the susceptibility …

Salinity is a constraint limiting plant growth and productivity of crops throughout the world.
Understanding the mechanism underlying plant response to salinity provides new insights …

Real-time RT-PCR is a powerful technique for the measurement of gene expression, but its
accuracy depends on the stability of the internal reference gene (s) used for data …

Tomato Yellow Leaf Curl Virus Disease incited by Tomato yellow leaf curl virus (TYLCV)
causes huge losses in tomato production worldwide and is caused by different related …

Molecular mechanisms associated with biochar-elicited suppression of soilborne plant
diseases and improved plant performance are not well understood. A stem base inoculation …

Background RT-qPCR is a preferred method for rapid and reliable quantification of gene
expression studies. Appropriate application of RT-qPCR in such studies requires the use of …

Quantitative real-time PCR (qPCR) is a powerful tool to measure gene expression levels.
Accurate and reproducible results are dependent on the correct choice of the reference …