Light-sheets and smart microscopy, an exciting future is dawning
S Daetwyler, RP Fiolka - Communications biology, 2023 - nature.com
Light-sheet fluorescence microscopy has transformed our ability to visualize and
quantitatively measure biological processes rapidly and over long time periods. In this …
quantitatively measure biological processes rapidly and over long time periods. In this …
Tutorial: practical considerations for tissue clearing and imaging
Tissue clearing has become a powerful technique for studying anatomy and morphology at
scales ranging from entire organisms to subcellular features. With the recent proliferation of …
scales ranging from entire organisms to subcellular features. With the recent proliferation of …
High cell density and high-resolution 3D bioprinting for fabricating vascularized tissues
Three-dimensional (3D) bioprinting techniques have emerged as the most popular methods
to fabricate 3D-engineered tissues; however, there are challenges in simultaneously …
to fabricate 3D-engineered tissues; however, there are challenges in simultaneously …
Volumetric bioprinting of organoids and optically tuned hydrogels to build liver‐like metabolic biofactories
PN Bernal, M Bouwmeester, J Madrid‐Wolff… - Advanced …, 2022 - Wiley Online Library
Organ‐and tissue‐level biological functions are intimately linked to microscale cell–cell
interactions and to the overarching tissue architecture. Together, biofabrication and …
interactions and to the overarching tissue architecture. Together, biofabrication and …
Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms
INTRODUCTION Organisms live by means of the complex, dynamic, three-dimensional (3D)
interplay between millions of components, from the molecular to the multicellular. Visualizing …
interplay between millions of components, from the molecular to the multicellular. Visualizing …
Controlling light in scattering materials for volumetric additive manufacturing
Abstract 3D printing has revolutionized the manufacturing of volumetric components and
structures in many areas. Several fully volumetric light‐based techniques have been …
structures in many areas. Several fully volumetric light‐based techniques have been …
Content-aware image restoration: pushing the limits of fluorescence microscopy
Fluorescence microscopy is a key driver of discoveries in the life sciences, with observable
phenomena being limited by the optics of the microscope, the chemistry of the fluorophores …
phenomena being limited by the optics of the microscope, the chemistry of the fluorophores …
Three-dimensional structured illumination microscopy with enhanced axial resolution
The axial resolution of three-dimensional structured illumination microscopy (3D SIM) is
limited to∼ 300 nm. Here we present two distinct, complementary methods to improve axial …
limited to∼ 300 nm. Here we present two distinct, complementary methods to improve axial …
Transcription organizes euchromatin via microphase separation
L Hilbert, Y Sato, K Kuznetsova, T Bianucci… - Nature …, 2021 - nature.com
In eukaryotes, DNA is packed inside the cell nucleus in the form of chromatin, which consists
of DNA, proteins such as histones, and RNA. Euchromatin, which is permissive for …
of DNA, proteins such as histones, and RNA. Euchromatin, which is permissive for …
A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics
We present an oblique plane microscope (OPM) that uses a bespoke glass-tipped tertiary
objective to improve the resolution, field of view, and usability over previous variants. Owing …
objective to improve the resolution, field of view, and usability over previous variants. Owing …