[HTML][HTML] Integrating cellular electron microscopy with multimodal data to explore biology across space and time

CL McCafferty, S Klumpe, RE Amaro, W Kukulski… - Cell, 2024 - cell.com
Biology spans a continuum of length and time scales. Individual experimental methods only
glimpse discrete pieces of this spectrum but can be combined to construct a more holistic …

[HTML][HTML] The Structure of Cilium Inner Junctions Revealed by Electron Cryo-tomography

S Li, JJ Fernandez, MD Ruehle, RA Howard-Till… - …, 2024 - pmc.ncbi.nlm.nih.gov
The cilium is a microtubule-based organelle critical for many cellular functions. Its assembly
initiates at a basal body and continues as an axoneme that projects out of the cell to form a …

[HTML][HTML] Structures of Native Doublet Microtubules from Trichomonas vaginalis Reveal Parasite-Specific Proteins as Potential Drug Targets

A Stevens, S Kashyap, EH Crofut, SE Wang… - Research …, 2024 - pmc.ncbi.nlm.nih.gov
Doublet microtubules (DMTs) are flagellar components required for the protist Trichomonas
vaginalis (Tv) to swim through the human genitourinary tract to cause trichomoniasis, the …

Structure of AcMNPV nucleocapsid reveals DNA portal organization and packaging apparatus of circular dsDNA baculovirus.

G Effantin, E Kandiah, M Pelosse - bioRxiv, 2024 - biorxiv.org
Baculoviruses are large DNA viruses found in nature propagating amongst insects and
lepidoptera in particular. Their viral cycle has been studied and characterized over decades …

In-cell structural insight into the asymmetric assembly of central apparatus in mammalian sperm axoneme

Y Zhu, G Yin, L Tai, B Wang, Y Lu, Z Zhang, SR Chen… - bioRxiv, 2024 - biorxiv.org
In motile cilia or flagella, the axoneme typically exhibits a" 9+ 2" configuration, with the
central apparatus (CA) consisting of extensively modified microtubules C1 and C2 to …

DiffFit: Differentiable Fitting of Molecule Structures to a Cryo-EM Map

D Luo, Z Alsuwaykit, D Khan, O Strnad… - ICML Workshop on …, 2024 - inria.hal.science
We introduce DiffFit, a differentiable algorithm for fitting protein atomistic structures into
experimental Cryo-Electron Microscopy (cryo-EM) volume maps. This process is crucial in …

Structures of Native Doublet Microtubules from Trichomonas vaginalis Reveal Parasite-Specific Proteins as Potential Drug Targets

ZH Zhou, A Stevens, S Kashyap, E Crofut, S Wang… - 2024 - researchsquare.com
Doublet microtubules (DMTs) are flagellar components required for the protist Trichomonas
vaginalis (Tv) to swim through the human genitourinary tract to cause trichomoniasis, the …