Essentially all cells contain a variety of spatially restricted regions that are important for
carrying out specialized functions. Often, these regions contain specialized transcriptomes …

Eukaryotes are divided into two major compartments: the nucleus where RNA is synthesized
and processed, and the cytoplasm, where mRNA is translated into proteins. Although many …

Local translation allows spatial control of gene expression. Here, we performed a dual
protein-mRNA localization screen, using smFISH on 523 human cell lines expressing GFP …

Background While numerous studies have described the transcriptomes of extracellular
vesicles (EVs) in different cellular contexts, these efforts have typically relied on sequencing …

The spatial organization of molecules in a cell is essential for their functions. While current
methods focus on discerning tissue architecture, cell–cell interactions, and spatial …

Although APEX2-mediated proximity labeling has been extensively implemented for
studying RNA subcellular localization in live cells, the biotin-phenoxyl radical used for …

Summary G3BP RNA-binding proteins are important components of stress granules (SGs).
Here, we analyze the role of the Drosophila G3BP Rasputin (RIN) in unstressed cells, where …

RNA localization and local translation are important for numerous cellular functions. In
mammals, a class of mRNAs localize to cytoplasmic protrusions in an APC-dependent …

Gene expression analysis requires accurate measurements of global RNA degradation
rates, earlier problematic with methods disruptive to cell physiology. Recently, metabolic …

Transport of mRNAs is an important step of gene expression, which brings the genetic
message from the DNA in the nucleus to a precise cytoplasmic location in a regulated …