[HTML][HTML] Environmental DNA metabarcoding for benthic monitoring: A review of sediment sampling and DNA extraction methods

J Pawlowski, K Bruce, K Panksep, FI Aguirre… - Science of the Total …, 2022 - Elsevier
Environmental DNA (eDNA) metabarcoding (parallel sequencing of DNA/RNA for
identification of whole communities within a targeted group) is revolutionizing the field of …

A practical guide to DNA metabarcoding for entomological ecologists

M Liu, LJ Clarke, SC Baker, GJ Jordan… - Ecological …, 2020 - Wiley Online Library
1. DNA metabarcoding is a cost‐effective species identification approach with great potential
to assist entomological ecologists. This review presents a practical guide to help …

Meta‐analysis shows both congruence and complementarity of DNA and eDNA metabarcoding to traditional methods for biological community assessment

F Keck, RC Blackman, R Bossart… - Molecular …, 2022 - Wiley Online Library
DNA metabarcoding is increasingly used for the assessment of aquatic communities, and
numerous studies have investigated the consistency of this technique with traditional …

MIDORI2: A collection of quality controlled, preformatted, and regularly updated reference databases for taxonomic assignment of eukaryotic mitochondrial sequences

M Leray, N Knowlton, RJ Machida - Environmental DNA, 2022 - Wiley Online Library
Abstract Analysis of environmental DNA is increasingly used to characterize ecological
communities, but the effectiveness of this approach depends on the accuracy of taxonomic …

eDNA metabarcoding as a promising conservation tool for monitoring fish diversity in a coastal wetland of the Pearl River Estuary compared to bottom trawling

K Zou, J Chen, H Ruan, Z Li, W Guo, M Li… - Science of the Total …, 2020 - Elsevier
The difficulty of censusing fish diversity hampers effective management and conservation in
estuarine and coastal ecosystems, especially wetland ecosystems. Improved noninvasive …

Comprehensive biodiversity analysis via ultra-deep patterned flow cell technology: a case study of eDNA metabarcoding seawater

GAC Singer, NA Fahner, JG Barnes, A McCarthy… - Scientific reports, 2019 - nature.com
The characterization of biodiversity is a crucial element of ecological investigations as well
as environmental assessment and monitoring activities. Increasingly, amplicon-based …

Marine biomonitoring with eDNA: Can metabarcoding of water samples cut it as a tool for surveying benthic communities?

A Antich, C Palacín, E Cebrian, R Golo… - Molecular …, 2021 - Wiley Online Library
In the marine realm, biomonitoring using environmental DNA (eDNA) of benthic
communities requires destructive direct sampling or the setting‐up of settlement structures …

Metabarcoding from microbes to mammals: comprehensive bioassessment on a global scale

ZG Compson, B McClenaghan, GAC Singer… - Frontiers in Ecology …, 2020 - frontiersin.org
Global biodiversity loss is unprecedented, and threats to existing biodiversity are growing.
Given pervasive global change, a major challenge facing resource managers is a lack of …

Monitoring non-indigenous species in port habitats: first application of a standardized North American protocol in the Mediterranean Sea

M Tamburini, E Keppel, A Marchini… - Frontiers in Marine …, 2021 - frontiersin.org
Baseline port monitoring for fouling communities is an essential tool to assess non-
indigenous species (NIS) introduction and spread, but a standardized and coordinated …

Beyond the visual: using metabarcoding to characterize the hidden reef cryptobiome

S Carvalho, E Aylagas, R Villalobos… - … of the Royal …, 2019 - royalsocietypublishing.org
In an era of coral reef degradation, our knowledge of ecological patterns in reefs is biased
towards large conspicuous organisms. The majority of biodiversity, however, inhabits small …