RNA is a key regulator of almost every cellular process, and the structures adopted by RNA
molecules are thought to be central to their functions. The recent fast-paced evolution of high …

Over the past decade, RNA sequencing (RNA-seq) has become an indispensable tool for
transcriptome-wide analysis of differential gene expression and differential splicing of …

Recent events have pushed RNA research into the spotlight. Continued discoveries of RNA
with unexpected diverse functions in healthy and diseased cells, such as the role of RNA as …

RNA molecules can fold into intricate shapes that can provide an additional layer of control
of gene expression beyond that of their sequence. In this Review, we discuss the current …

Long noncoding RNAs (lncRNAs) have emerged as significant players in almost every level
of gene function and regulation. Thus, characterizing the structures and interactions of …

SARS-CoV-2 carries the largest single-stranded RNA genome and is the causal pathogen of
the ongoing COVID-19 pandemic. How the SARS-CoV-2 RNA genome is folded in the virion …

Coupling of structure-specific in vivo chemical modification to next-generation sequencing is
transforming RNA secondary structure studies in living cells. The dominant strategy for …

Guanine (G)-rich sequences in nucleic acids can assemble into G-quadruplex structures that
involve G-quartets linked by loop nucleotides. The structural and topological diversity of G …

We introduce RNA G-quadruplex sequencing (rG4-seq), a transcriptome-wide RNA G-
quadruplex (rG4) profiling method that couples rG4-mediated reverse transcriptase stalling …

Selective 2′-hydroxyl acylation analyzed by primer extension (SHAPE) chemistries exploit
small electrophilic reagents that react with 2′-hydroxyl groups to interrogate RNA structure …