Post-translational modifications (PTMs) produce significant changes in the structural
properties of intrinsically disordered proteins (IDPs) by affecting their energy landscapes …

▪ Abstract Expressed protein ligation (EPL) is a protein engineering approach that allows
recombinant and synthetic polypeptides to be chemoselectively and regioselectively joined …

The coronavirus disease pandemic of 2019 (COVID-19) caused by the novel SARS-CoV-2
coronavirus resulted in economic losses and threatened human health worldwide. The …

Cytotoxic T lymphocytes (CTLs) detect and destroy cells displaying class I molecules of the
major histocompatibility complex (MHC) that present oligopeptides derived from aberrant …

▪ Abstract Protein splicing is a form of posttranslational processing that consists of the
excision of an intervening polypeptide sequence, the intein, from a protein, accompanied by …

Aims This review is intended to update our previous discussion on the chemoenzymatic
synthesis of carbohydrates and glycoconjugates,(1) with new emphasis placed on …

Biophysical techniques such as fluorescence spectroscopy and nuclear magnetic
resonance (NMR) spectroscopy provide a window into the inner workings of proteins. These …

SpyTag is a peptide that spontaneously forms an amide bond with its protein partner
SpyCatcher. SpyTag was fused at the N terminus of β‐lactamase and SpyCatcher at the C …

Protein splicing, the protein equivalent of RNA splicing, is a newly discovered
posttranslational process that proceeds through a branched protein intermediate and …

The determinants governing the self-catalyzed splicing and cleavage events by a mini-intein
of 154 amino acids, derived from the dnaB gene of Synechocystis sp. were investigated. The …