X-ray crystallography enables detailed structural studies of proteins to understand and
modulate their function. Conducting crystallographic experiments at cryogenic temperatures …

With recent developments in X-ray sources, instrumentation and data-analysis tools, time-
resolved crystallographic experiments, which were originally the preserve of a few expert …

Targeting protein kinases is an important strategy for intervention in cancer. Inhibitors are
directed at the active conformation or a variety of inactive conformations. While attempts …

The enzyme soybean lipoxygenase (SLO) provides a prototype for deep tunneling
mechanisms in hydrogen transfer catalysis. This work combines room temperature X-ray …

While protein conformational heterogeneity plays an important role in many aspects of
biological function, including ligand binding, its impact has been difficult to quantify …

Allostery is an inherent feature of proteins, but it remains challenging to reveal the
mechanisms by which allosteric signals propagate. A clearer understanding of this intrinsic …

Determining the interconverting conformations of dynamic proteins in atomic detail is a
major challenge for structural biology. Conformational heterogeneity in the active site of the …

Synonymous codons translate into chemically identical amino acids. Once considered
inconsequential to the formation of the protein product, there is evidence to suggest that …

Rational design and directed evolution have proved to be successful approaches to
increase catalytic efficiencies of both natural and artificial enzymes. Protein dynamics is …

Decades of structure-function studies have established our current extensive understanding
of enzymes. However, traditional structural models are snapshots of broader conformational …