CRISPR-Cas9 system: A new-fangled dawn in gene editing
Till date, only three techniques namely Zinc Finger Nuclease (ZFN), Transcription-Activator
Like Effector Nucleases (TALEN) and Clustered Regularly Interspaced Short Palindromic …
Like Effector Nucleases (TALEN) and Clustered Regularly Interspaced Short Palindromic …
[HTML][HTML] CRISPR/Cas-mediated plant genome editing: outstanding challenges a decade after implementation
The discovery of the CRISPR/Cas genome-editing system has revolutionized our
understanding of the plant genome. CRISPR/Cas has been used for over a decade to …
understanding of the plant genome. CRISPR/Cas has been used for over a decade to …
Genome editing of the disease susceptibility gene CsLOB1 in citrus confers resistance to citrus canker
Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces
many biotic challenges, including bacterial canker and Huanglongbing (HLB). Breeding for …
many biotic challenges, including bacterial canker and Huanglongbing (HLB). Breeding for …
Modification of the PthA4 effector binding elements in Type I Cs LOB 1 promoter using Cas9/sg RNA to produce transgenic Duncan grapefruit alleviating XccΔpthA4 …
Citrus canker caused by Xanthomonas citri subspecies citri (Xcc) is a severe disease for
most commercial citrus cultivars and responsible for significant economic losses worldwide …
most commercial citrus cultivars and responsible for significant economic losses worldwide …
CRISPR‐LbCas12a‐mediated modification of citrus
Summary Recently, CRISPR‐Cas12a (Cpf1) from Prevotella and Francisella was
engineered to modify plant genomes. In this report, we employed CRISPR‐LbCas12a …
engineered to modify plant genomes. In this report, we employed CRISPR‐LbCas12a …
[HTML][HTML] CRISPR/Cas genome editing technologies for plant improvement against biotic and abiotic stresses: advances, limitations, and future perspectives
Y Wang, N Zafar, Q Ali, H Manghwar, G Wang, L Yu… - Cells, 2022 - mdpi.com
Crossbreeding, mutation breeding, and traditional transgenic breeding take much time to
improve desirable characters/traits. CRISPR/Cas-mediated genome editing (GE) is a game …
improve desirable characters/traits. CRISPR/Cas-mediated genome editing (GE) is a game …
Current and future editing reagent delivery systems for plant genome editing
Many genome editing tools have been developed and new ones are anticipated; some have
been extensively applied in plant genetics, biotechnology and breeding, especially the …
been extensively applied in plant genetics, biotechnology and breeding, especially the …
[HTML][HTML] Highly efficient generation of canker-resistant sweet orange enabled by an improved CRISPR/Cas9 system
Sweet orange (Citrus sinensis) is the most economically important species for the citrus
industry. However, it is susceptible to many diseases including citrus bacterial canker …
industry. However, it is susceptible to many diseases including citrus bacterial canker …
Biallelic Editing of the LOB1 Promoter via CRISPR/Cas9 Creates Canker-Resistant 'Duncan' Grapefruit
Citrus canker caused by Xanthomonas citri subsp. citri is one of the most devastating citrus
diseases worldwide. Generating disease-resistant citrus varieties is considered one of the …
diseases worldwide. Generating disease-resistant citrus varieties is considered one of the …
[HTML][HTML] Sweet Orange: Evolution, characterization, varieties, and breeding perspectives
Among Citrus species, the sweet orange (Citrus sinensis (L.) Osbeck) is the most important
in terms of production volumes and cultivated areas. Oranges are particularly appreciated …
in terms of production volumes and cultivated areas. Oranges are particularly appreciated …