The construction of custom libraries is critical for rational protein engineering and directed
evolution. Array‐synthesized oligo pools of thousands of user‐defined sequences (up to∼ …

Highlights•CRISPR activation is an emerging tool for engineering bacterial metabolism.•The
promoter design rules for effective CRISPR activation in bacteria are complex.•Guide RNAs …

For decades, fungi have been a source of US Food and Drug Administration–approved
natural products such as penicillin, cyclosporine, and the statins. Recent breakthroughs in …

Our understanding of how genotype controls phenotype is limited by the scale at which we
can precisely alter the genome and assess the phenotypic consequences of each …

Monoterpene indole alkaloids (MIAs) are an expansive class of plant natural products, many
of which have been named on the World Health Organization's List of Essential Medicines …

Genome-scale CRISPR interference (CRISPRi) is widely utilized to study cellular processes
in a variety of organisms. Despite the dominance of Saccharomyces cerevisiae as a model …

As an important cell factory, industrial yeast has been widely used for the production of
compounds ranging from bulk chemicals to complex natural products. However, various …

Deciphering gene function is fundamental to engineering of microbiology. The clustered
regularly interspaced short palindromic repeats (CRISPR) system has been adapted for …

The CRISPR/Cas9 systems have been developed as tools for genetic engineering and
metabolic engineering in various organisms. In this review, various aspects of CRISPR/Cas9 …

Abstract The engineering of Clustered Regularly Interspaced Short Palindromic Repeats
(CRISPR)-CRISPR-associated proteins continues to expand the toolkit available for genome …