Single-molecule localization microscopy

M Lelek, MT Gyparaki, G Beliu, F Schueder… - Nature reviews …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Visualizing and discovering cellular structures with super-resolution microscopy

YM Sigal, R Zhou, X Zhuang - Science, 2018 - science.org
Super-resolution microscopy has overcome a long-held resolution barrier—the diffraction
limit—in light microscopy and enabled visualization of previously invisible molecular details …

Deep learning enables cross-modality super-resolution in fluorescence microscopy

H Wang, Y Rivenson, Y Jin, Z Wei, R Gao… - Nature …, 2019 - nature.com
We present deep-learning-enabled super-resolution across different fluorescence
microscopy modalities. This data-driven approach does not require numerical modeling of …

Applications, promises, and pitfalls of deep learning for fluorescence image reconstruction

C Belthangady, LA Royer - Nature methods, 2019 - nature.com
Deep learning is becoming an increasingly important tool for image reconstruction in
fluorescence microscopy. We review state-of-the-art applications such as image restoration …

Deep-STORM: super-resolution single-molecule microscopy by deep learning

E Nehme, LE Weiss, T Michaeli, Y Shechtman - Optica, 2018 - opg.optica.org
We present an ultrafast, precise, parameter-free method, which we term Deep-STORM, for
obtaining super-resolution images from stochastically blinking emitters, such as fluorescent …

Super-resolution microscopy with DNA-PAINT

J Schnitzbauer, MT Strauss, T Schlichthaerle… - Nature protocols, 2017 - nature.com
Super-resolution techniques have begun to transform biological and biomedical research by
allowing researchers to observe structures well below the classic diffraction limit of light …

Deep learning massively accelerates super-resolution localization microscopy

W Ouyang, A Aristov, M Lelek, X Hao, C Zimmer - Nature biotechnology, 2018 - nature.com
The speed of super-resolution microscopy methods based on single-molecule localization,
for example, PALM and STORM, is limited by the need to record many thousands of frames …

Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software

D Sage, TA Pham, H Babcock, T Lukes, T Pengo… - Nature …, 2019 - nature.com
With the widespread uptake of two-dimensional (2D) and three-dimensional (3D) single-
molecule localization microscopy (SMLM), a large set of different data analysis packages …

Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations

N Gustafsson, S Culley, G Ashdown, DM Owen… - Nature …, 2016 - nature.com
Despite significant progress, high-speed live-cell super-resolution studies remain limited to
specialized optical setups, generally requiring intense phototoxic illumination. Here, we …

Three-dimensional localization of single molecules for super-resolution imaging and single-particle tracking

L von Diezmann, Y Shechtman, WE Moerner - Chemical reviews, 2017 - ACS Publications
Single-molecule super-resolution fluorescence microscopy and single-particle tracking are
two imaging modalities that illuminate the properties of cells and materials on spatial scales …