Structural biologists have traditionally approached cellular complexity in a reductionist
manner in which the cellular molecular components are fractionated and purified before …

Recent advances in cryo-electron microscopy have marked only the beginning of the
potential of this technique. To bring structure into cell biology, the modality of cryo-electron …

We present a new approach for macromolecular structure determination from multiple
particles in electron cryo-tomography (cryo-ET) data sets. Whereas existing subtomogram …

In situ cryo electron tomography of cryo focused ion beam milled samples has emerged in
recent years as a powerful technique for structural studies of macromolecular complexes in …

Single-particle cryo-electron tomography is an emerging technique capable of determining
the structure of proteins imaged within the native context of cells at molecular resolution …

Cryogenic electron tomography (cryoET) allows visualization of cellular structures in situ.
However, anisotropic resolution arising from the intrinsic “missing-wedge” problem has …

Cryo-electron microscopy (cryo-EM) enables macromolecular structure determination in vitro
and inside cells. In addition to aligning individual particles, accurate registration of sample …

The acquisition of cryo-electron microscopy (cryo-EM) data from biological specimens must
be tightly coupled to data preprocessing to ensure the best data quality and microscope …

Imaging large fields of view while preserving high-resolution structural information remains a
challenge in low-dose cryo-electron tomography. Here we present robust tools for montage …

Highlights•Structural biology plays a vital role in SARS-CoV-2 vaccine and treatment.•High-
resolution structures of SARS-CoV-2 proteins and complexes have been obtained.•In situ …