As interest in space exploration rises, there is a growing need to quantify the impact of
microgravity on the growth, survival, and adaptation of microorganisms, including those …

Distinct cellular functions are executed by separate groups of proteins, organized into
complexes or functional modules, which are ultimately interconnected in cell-wide protein …

Efforts to map the Escherichia coli interactome have identified several hundred
macromolecular complexes, but direct binary protein-protein interactions (PPIs) have not …

Comparative genomics is the cornerstone of identification of gene functions. The immense
number of living organisms precludes experimental identification of functions except in a …

One-third of the 4,225 protein-coding genes of Escherichia coli K-12 remain functionally
unannotated (orphans). Many map to distant clades such as Archaea, suggesting …

Bacteria and Archaea display a variety of phenotypic traits and can adapt to diverse
ecological niches. However, systematic annotation of prokaryotic phenotypes is lacking. We …

The cytoskeleton has a key function in the temporal and spatial organization of both
prokaryotic and eukaryotic cells. Here, we report the identification of a new class of polymer …

Background Yeast two-hybrid (Y2H) screens have been among the most powerful methods
to detect and analyze protein-protein interactions. However, they suffer from a significant …

Campylobacter jejuni is the leading cause of infectious gastroenteritis in industrialized
nations. Its ability to enter and survive within nonphagocytic cells is thought to be very …

Yeast two-hybrid (Y2H) screening methods are an effective means for the detection of
protein–protein interactions. Optimisation and automation has increased the throughput of …