A guide to light-sheet fluorescence microscopy for multiscale imaging
RM Power, J Huisken - Nature methods, 2017 - nature.com
The impact of light-sheet fluorescence microscopy (LSFM) is visible in fields as diverse as
developmental and cell biology, anatomical science, biophysics and neuroscience …
developmental and cell biology, anatomical science, biophysics and neuroscience …
Light-sheet microscopy: a tutorial
This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from
an optics perspective. As such, emphasis is placed on the advantages that LS microscope …
an optics perspective. As such, emphasis is placed on the advantages that LS microscope …
The light-sheet microscopy revolution
JM Girkin, MT Carvalho - Journal of Optics, 2018 - iopscience.iop.org
This paper reviews the rapid advances that have been made in one form of optical biological
imaging in the last decade, namely that of light sheet microscopy. Although the concept was …
imaging in the last decade, namely that of light sheet microscopy. Although the concept was …
Instantaneous isotropic volumetric imaging of fast biological processes
N Wagner, N Norlin, J Gierten, G De Medeiros… - Nature …, 2019 - nature.com
To capture highly dynamic biological processes at cellular resolution is a recurring
challenge in biology. Here we show that combining selective-volume illumination with …
challenge in biology. Here we show that combining selective-volume illumination with …
How to define and optimize axial resolution in light-sheet microscopy: a simulation-based approach
E Remacha, L Friedrich, J Vermot… - Biomedical optics …, 2019 - opg.optica.org
“How thick is your light sheet?” is a question that has been asked frequently after talks
showing impressive renderings of 3D data acquired by a light-sheet microscope. This …
showing impressive renderings of 3D data acquired by a light-sheet microscope. This …
Light sheet approaches for improved precision in 3D localization-based super-resolution imaging in mammalian cells
The development of imaging techniques beyond the diffraction limit has paved the way for
detailed studies of nanostructures and molecular mechanisms in biological systems …
detailed studies of nanostructures and molecular mechanisms in biological systems …
Breaking the Axial Diffraction Limit: A Guide to Axial Super‐Resolution Fluorescence Microscopy
Optical microscopy is a powerful tool for understanding the fundamentals of the microscopic
world. However, for centuries its resolving ability remained limited by the optical diffraction …
world. However, for centuries its resolving ability remained limited by the optical diffraction …
Three-dimensional single-molecule localization microscopy in whole-cell and tissue specimens
Super-resolution microscopy techniques are versatile and powerful tools for visualizing
organelle structures, interactions, and protein functions in biomedical research. However …
organelle structures, interactions, and protein functions in biomedical research. However …
Speed limits of structured illumination microscopy
F Ströhl, CF Kaminski - Optics letters, 2017 - opg.optica.org
A theoretical framework for widefield structured illumination microscopy (SIM) reconstruction
from fewer than the commonly used nine raw frame acquisitions is introduced and applied in …
from fewer than the commonly used nine raw frame acquisitions is introduced and applied in …
Answering some questions about structured illumination microscopy
JD Manton - … Transactions of the Royal Society A, 2022 - royalsocietypublishing.org
Structured illumination microscopy (SIM) provides images of fluorescent objects at an
enhanced resolution greater than that of conventional epifluorescence wide-field …
enhanced resolution greater than that of conventional epifluorescence wide-field …