Advancements in mammalian cell transient gene expression (TGE) technology for accelerated production of biologics

S Gutiérrez-Granados, L Cervera… - Critical reviews in …, 2018 - Taylor & Francis
Transient gene expression (TGE) in animal cell cultures has been used for almost 30 years
to produce milligrams and grams of recombinant proteins, virus-like particles and viral …

Yeast as an expression system for producing virus‐like particles: what factors do we need to consider?

HJ Kim, HJ Kim - Letters in applied microbiology, 2017 - academic.oup.com
The development of various types of virus‐like particles (VLPs) has accelerated over the
past two decades as the importance of VLPs for generating next‐generation vaccines has …

NAD (P)-dependent glucose dehydrogenase: Applications for biosensors, bioelectrodes, and biofuel cells

K Stolarczyk, J Rogalski, R Bilewicz - Bioelectrochemistry, 2020 - Elsevier
This review discusses the physical and chemical properties of nicotinamide redox cofactor
dependent glucose dehydrogenase (NAD (P) dependent GDH) and its extensive application …

Elevated microRNA-181c and microRNA-30d levels in the enlarged amygdala of the valproic acid rat model of autism

NFMO Loohuis, K Kole, JC Glennon, P Karel… - Neurobiology of …, 2015 - Elsevier
Autism spectrum disorders are severe neurodevelopmental disorders, marked by
impairments in reciprocal social interaction, delays in early language and communication …

Hepatic overexpression of hemopexin inhibits inflammation and vascular stasis in murine models of sickle cell disease

GM Vercellotti, P Zhang, J Nguyen, F Abdulla… - Molecular …, 2016 - Springer
Sickle cell disease (SCD) patients have low serum hemopexin (Hpx) levels due to chronic
hemolysis. We hypothesized that in SCD mice, hepatic overexpression of hemopexin would …

C1QL3 promotes cell‐cell adhesion by mediating complex formation between ADGRB3/BAI3 and neuronal pentraxins

MJ Sticco, PA Peña Palomino… - The FASEB …, 2021 - Wiley Online Library
Synapses are the fundamental structural unit by which neurons communicate. An orchestra
of proteins regulates diverse synaptic functions, including synapse formation, maintenance …

Conformational plasticity of the HIV-1 gp41 immunodominant region is recognized by multiple non-neutralizing antibodies

JD Cook, A Khondker, JE Lee - Communications Biology, 2022 - nature.com
The early humoral immune response to acute HIV-1 infection is largely non-neutralizing. The
principal target of these antibodies is the primary immunodominant region (PID) on the gp41 …

Interaction of axonal chondrolectin with collagen XIXa1 is necessary for precise neuromuscular junction formation

AM Oprişoreanu, HL Smith, S Arya, R Webster… - Cell Reports, 2019 - cell.com
Chondrolectin (Chodl) is needed for motor axon extension in zebrafish and is dysregulated
in mouse models of spinal muscular atrophy (SMA). However, the mechanistic basis of …

Efficient production and purification of recombinant human interleukin-12 (IL-12) overexpressed in mammalian cells without affinity tag

S Jayanthi, B prasanth Koppolu, SG Smith… - Protein expression and …, 2014 - Elsevier
Interleukin-12 is a heterodimeric, pro-inflammatory cytokine that is a key driver of cell-
mediated immunity. Clinical interest in IL-12 is significant due to its potent anti-tumor activity …

Construction of a eukaryotic expression system with stable and secretory expression of Mycobacterium tuberculosis 38 kDa protein

H Chen, Z Chen, N Bai, R Yan, M Xu, W Wu… - World Journal of …, 2021 - Springer
The 38 kDa protein is a major antigen of mycobacterium tuberculosis and has been widely
used in TB serodiagnosis, due to its highly sensitivity and specificity. Here we attempt to …