In contrast to Gram-negative bacteria, secretory proteins of Gram-positive bacteria only need
to traverse a single membrane to enter the extracellular environment. For this reason, Gram …

The major route of protein translocation in bacteria is the so-called general secretion
pathway (Sec-pathway). This route has been extensively studied in Escherichia coli and …

The SecA subunit of E. coli preprotein translocase promotes protein secretion during cycles
of membrane insertion and deinsertion at SecYEG. This process is regulated both by …

SecA is the ATP‐dependent force generator in the Escherichia coli precursor protein
translocation cascade, and is bound at the membrane surface to the integral membrane …

Six putative ATP‐binding motifs of SecA protein were altered by oligonucleotide‐directed
mutagenesis to try to define the ATP‐binding regions of this multifunctional protein. The …

Vibrio cholerae secretes a number of proteins important for virulence, including cholera
toxin. This process requires the products of the eps genes which have homologues in …

Revised Manuscript Received August 30, 1993® abstract: SecA, the peripheral ATPase
domain of the Escherichia coli precursor protein translocase, was denatured in 6 M …

Most secretory proteins that are destined for the periplasm or the outer membrane are
exported through the bacterial plasma membrane by the Sec translocase. Translocase is a …

E. coli cells harboring pCG169 containing the secD secF locus possessed SecA protein
almost entirely in an integral membrane form in which it displayed normal protein …

Bacillus subtilis is one of the best-studied organisms. Due to the broad knowledge and
annotation and the well-developed genetic system, this bacterium is an excellent starting …