Macromolecular crowding, phase separation, and homeostasis in the orchestration of bacterial cellular functions

B Monterroso, W Margolin, AJ Boersma, G Rivas… - Chemical …, 2024 - ACS Publications
Macromolecular crowding affects the activity of proteins and functional macromolecular
complexes in all cells, including bacteria. Crowding, together with physicochemical …

Macromolecular crowding: Sensing without a sensor

LJ Holt, M Delarue - Current Opinion in Cell Biology, 2023 - Elsevier
All living cells are crowded with macromolecules. Crowding can directly modulate
biochemical reactions to various degrees depending on the sizes, shapes, and binding …

Cell cycle-coordinated maintenance of the Vibrio bipartite genome

T Niault, J Czarnecki, M Lambérioux, D Mazel… - EcoSal …, 2023 - Am Soc Microbiol
To preserve the integrity of their genome, bacteria rely on several genome maintenance
mechanisms that are co-ordinated with the cell cycle. All members of the Vibrio family have a …

Fundamental limits on the rate of bacterial growth and their influence on proteomic composition

NM Belliveau, G Chure, CL Hueschen, HG Garcia… - Cell Systems, 2021 - cell.com
Despite abundant measurements of bacterial growth rate, cell size, and protein content, we
lack a rigorous understanding of what sets the scale of these quantities and when protein …

Chromosomal position of ribosomal protein genes affects long-term evolution of Vibrio cholerae

L Larotonda, D Mornico, V Khanna, J Bernal-Bayard… - Mbio, 2023 - Am Soc Microbiol
It is unclear how gene order within the chromosome influences genome evolution. Bacteria
cluster transcription and translation genes close to the replication origin (oriC). In Vibrio …

The coordinated replication of Vibrio cholerae's two chromosomes required the acquisition of a unique domain by the RctB initiator

F Fournes, T Niault, J Czarnecki… - Nucleic acids …, 2021 - academic.oup.com
Vibrio cholerae, the pathogenic bacterium that causes cholera, has two chromosomes
(Chr1, Chr2) that replicate in a well-orchestrated sequence. Chr2 initiation is triggered only …

Rationally designed chromosome fusion does not prevent rapid growth of Vibrio natriegens

L Ramming, D Stukenberg, MC Sánchez Olmos… - Communications …, 2024 - nature.com
DNA replication is essential for the proliferation of all cells. Bacterial chromosomes are
replicated bidirectionally from a single origin of replication, with replication proceeding at …

Patterns of abundance, chromosomal localization, and domain organization among c-di-GMP-metabolizing genes revealed by comparative genomics of five …

S Koppenhöfer, AS Lang - BMC genomics, 2022 - Springer
Abstract Background Bis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) is
a bacterial second messenger that affects diverse processes in different bacteria, including …

The distinct cell physiology of Bradyrhizobium at the population and cellular level

IF Medici, L Bartrolí, FF Guaimas, FR Fulgenzi… - BMC microbiology, 2024 - Springer
The α-Proteobacteria belonging to Bradyrhizobium genus are microorganisms of extreme
slow growth. Despite their extended use as inoculants in soybean production, their …

Vibrionaceae core, shell and cloud genes are non-randomly distributed on Chr 1: An hypothesis that links the genomic location of genes with their intracellular …

CB Sonnenberg, T Kahlke, P Haugen - BMC genomics, 2020 - Springer
Abstract Background The genome of Vibrionaceae bacteria, which consists of two circular
chromosomes, is replicated in a highly ordered fashion. In fast-growing bacteria, multifork …