Optical tweezers in single-molecule biophysics
CJ Bustamante, YR Chemla, S Liu… - Nature Reviews Methods …, 2021 - nature.com
Optical tweezers have become the method of choice in single-molecule manipulation
studies. In this Primer, we first review the physical principles of optical tweezers and the …
studies. In this Primer, we first review the physical principles of optical tweezers and the …
The AAA+ superfamily: a review of the structural and mechanistic principles of these molecular machines
ATPases associated with diverse cellular activities (AAA+ proteins) are a superfamily of
proteins found throughout all domains of life. The hallmark of this family is a conserved …
proteins found throughout all domains of life. The hallmark of this family is a conserved …
Substrate-engaged 26S proteasome structures reveal mechanisms for ATP-hydrolysis–driven translocation
INTRODUCTION As the major protease in eukaryotic cells and the final component of the
ubiquitin-proteasome system, the 26 S proteasome is responsible for protein homeostasis …
ubiquitin-proteasome system, the 26 S proteasome is responsible for protein homeostasis …
The molecular principles governing the activity and functional diversity of AAA+ proteins
ATPases associated with diverse cellular activities (AAA+ proteins) are macromolecular
machines that convert the chemical energy contained in ATP molecules into powerful …
machines that convert the chemical energy contained in ATP molecules into powerful …
Mechanistic insights into bacterial AAA+ proteases and protein-remodelling machines
AO Olivares, TA Baker, RT Sauer - Nature Reviews Microbiology, 2016 - nature.com
To maintain protein homeostasis, AAA+ proteolytic machines degrade damaged and
unneeded proteins in bacteria, archaea and eukaryotes. This process involves the ATP …
unneeded proteins in bacteria, archaea and eukaryotes. This process involves the ATP …
Smarca4 ATPase mutations disrupt direct eviction of PRC1 from chromatin
Trithorax-group proteins and their mammalian homologs, including those in BAF
(mSWI/SNF) complexes, are known to oppose the activity of Polycomb repressive …
(mSWI/SNF) complexes, are known to oppose the activity of Polycomb repressive …
Regulated protein turnover: snapshots of the proteasome in action
The ubiquitin proteasome system (UPS) is the main ATP-dependent protein degradation
pathway in the cytosol and nucleus of eukaryotic cells. At its centre is the 26S proteasome …
pathway in the cytosol and nucleus of eukaryotic cells. At its centre is the 26S proteasome …
Structures of the ATP-fueled ClpXP proteolytic machine bound to protein substrate
ClpXP is an ATP-dependent protease in which the ClpX AAA+ motor binds, unfolds, and
translocates specific protein substrates into the degradation chamber of ClpP. We present …
translocates specific protein substrates into the degradation chamber of ClpP. We present …
The role of single-protein elasticity in mechanobiology
AEM Beedle, S Garcia-Manyes - Nature Reviews Materials, 2023 - nature.com
In addition to biochemical signals and genetic considerations, mechanical forces are rapidly
emerging as a master regulator of human physiology. However, the molecular mechanisms …
emerging as a master regulator of human physiology. However, the molecular mechanisms …
Cooperation of Hsp70 and Hsp100 chaperone machines in protein disaggregation
A Mogk, E Kummer, B Bukau - Frontiers in molecular biosciences, 2015 - frontiersin.org
Unicellular and sessile organisms are particularly exposed to environmental stress such as
heat shock causing accumulation and aggregation of misfolded protein species. To …
heat shock causing accumulation and aggregation of misfolded protein species. To …