CRISPR-Cas9 system: A new-fangled dawn in gene editing
Till date, only three techniques namely Zinc Finger Nuclease (ZFN), Transcription-Activator
Like Effector Nucleases (TALEN) and Clustered Regularly Interspaced Short Palindromic …
Like Effector Nucleases (TALEN) and Clustered Regularly Interspaced Short Palindromic …
Disruption of Intracellular Calcium Homeostasis as a Therapeutic Target Against Trypanosoma cruzi
There is no effective cure for Chagas disease, which is caused by infection with the
arthropod-borne parasite, Trypanosoma cruzi. In the search for new drugs to treat Chagas …
arthropod-borne parasite, Trypanosoma cruzi. In the search for new drugs to treat Chagas …
A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids
T Beneke, R Madden, L Makin… - Royal Society …, 2017 - royalsocietypublishing.org
Clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR-associated
gene 9 (Cas9) genome editing is set to revolutionize genetic manipulation of pathogens …
gene 9 (Cas9) genome editing is set to revolutionize genetic manipulation of pathogens …
Expanding the toolbox for Trypanosoma cruzi: A parasite line incorporating a bioluminescence-fluorescence dual reporter and streamlined CRISPR/Cas9 functionality for rapid …
FC Costa, AF Francisco, S Jayawardhana… - PLoS neglected …, 2018 - journals.plos.org
Background Infection with Trypanosoma cruzi causes Chagas disease, a major public
health problem throughout Latin America. There is no vaccine and the only drugs have …
health problem throughout Latin America. There is no vaccine and the only drugs have …
Rapid, selection-free, high-efficiency genome editing in protozoan parasites using CRISPR-Cas9 ribonucleoproteins
Trypanosomatids (order Kinetoplastida), including the human pathogens Trypanosoma cruzi
(agent of Chagas disease), Trypanosoma brucei,(African sleeping sickness), and …
(agent of Chagas disease), Trypanosoma brucei,(African sleeping sickness), and …
Advances in the cellular biology, biochemistry, and molecular biology of acidocalcisomes
R Docampo - Microbiology and Molecular Biology Reviews, 2024 - Am Soc Microbiol
Acidocalcisomes are organelles conserved during evolution and closely related to the so-
called volutin granules of bacteria and archaea, to the acidocalcisome-like vacuoles of …
called volutin granules of bacteria and archaea, to the acidocalcisome-like vacuoles of …
CRISPR in parasitology: not exactly cut and dried!
CRISPR/Cas9 technology has been developing rapidly in the field of parasitology, allowing
for the dissection of molecular processes with unprecedented efficiency. Optimization and …
for the dissection of molecular processes with unprecedented efficiency. Optimization and …
IP3 receptor-mediated Ca2+ release from acidocalcisomes regulates mitochondrial bioenergetics and prevents autophagy in Trypanosoma cruzi
In contrast to animal cells, the inositol 1, 4, 5-trisphosphate receptor of Trypanosoma cruzi
(TcIP 3 R) localizes to acidocalcisomes instead of the endoplasmic reticulum. Here, we …
(TcIP 3 R) localizes to acidocalcisomes instead of the endoplasmic reticulum. Here, we …
LeishGEdit: a method for rapid gene knockout and tagging using CRISPR-Cas9
Postgenomic analyses of Leishmania biology benefit from rapid and precise methods for
gene manipulation. Traditional methods of gene knockout or tagging by homologous …
gene manipulation. Traditional methods of gene knockout or tagging by homologous …
Different Roles of Mitochondrial Calcium Uniporter Complex Subunits in Growth and Infectivity of Trypanosoma cruzi
MA Chiurillo, N Lander, MS Bertolini, M Storey… - MBio, 2017 - Am Soc Microbiol
Trypanosoma cruzi is the agent of Chagas disease, and the finding that this parasite
possesses a mitochondrial calcium uniporter (TcMCU) with characteristics similar to that of …
possesses a mitochondrial calcium uniporter (TcMCU) with characteristics similar to that of …