Single‐cell analysis using droplet microfluidics
Droplet microfluidics has revolutionized the study of single cells. The ability to
compartmentalize cells within picoliter droplets in microfluidic devices has opened up a wide …
compartmentalize cells within picoliter droplets in microfluidic devices has opened up a wide …
Approximation and inference methods for stochastic biochemical kinetics—a tutorial review
D Schnoerr, G Sanguinetti… - Journal of Physics A …, 2017 - iopscience.iop.org
Stochastic fluctuations of molecule numbers are ubiquitous in biological systems. Important
examples include gene expression and enzymatic processes in living cells. Such systems …
examples include gene expression and enzymatic processes in living cells. Such systems …
Challenges in measuring and understanding biological noise
Biochemical reactions are intrinsically stochastic, leading to variation in the production of
mRNAs and proteins within cells. In the scientific literature, this source of variation is typically …
mRNAs and proteins within cells. In the scientific literature, this source of variation is typically …
Droplet microfluidics—A tool for single‐cell analysis
HN Joensson… - Angewandte Chemie …, 2012 - Wiley Online Library
Droplet microfluidics allows the isolation of single cells and reagents in monodisperse
picoliter liquid capsules and manipulations at a throughput of thousands of droplets per …
picoliter liquid capsules and manipulations at a throughput of thousands of droplets per …
Nature, nurture, or chance: stochastic gene expression and its consequences
A Raj, A Van Oudenaarden - Cell, 2008 - cell.com
Gene expression is a fundamentally stochastic process, with randomness in transcription
and translation leading to cell-to-cell variations in mRNA and protein levels. This variation …
and translation leading to cell-to-cell variations in mRNA and protein levels. This variation …
The ground state of embryonic stem cell self-renewal
In the three decades since pluripotent mouse embryonic stem (ES) cells were first described,
they have been derived and maintained by using various empirical combinations of feeder …
they have been derived and maintained by using various empirical combinations of feeder …
Non-genetic origins of cell-to-cell variability in TRAIL-induced apoptosis
In microorganisms, noise in gene expression gives rise to cell-to-cell variability in protein
concentrations,,,,,,. In mammalian cells, protein levels also vary,, and individual cells differ …
concentrations,,,,,,. In mammalian cells, protein levels also vary,, and individual cells differ …
Single-cell proteomic analysis of S. cerevisiae reveals the architecture of biological noise
JRS Newman, S Ghaemmaghami, J Ihmels… - Nature, 2006 - nature.com
A major goal of biology is to provide a quantitative description of cellular behaviour. This
task, however, has been hampered by the difficulty in measuring protein abundances and …
task, however, has been hampered by the difficulty in measuring protein abundances and …
Bet hedging in yeast by heterogeneous, age-correlated expression of a stress protectant
Genetically identical cells grown in the same culture display striking cell-to-cell
heterogeneity in gene expression and other traits. A crucial challenge is to understand how …
heterogeneity in gene expression and other traits. A crucial challenge is to understand how …
Noise in protein expression scales with natural protein abundance
A Bar-Even, J Paulsson, N Maheshri, M Carmi… - Nature …, 2006 - nature.com
Noise in gene expression is generated at multiple levels, such as transcription and
translation, chromatin remodeling and pathway-specific regulation. Studies of individual …
translation, chromatin remodeling and pathway-specific regulation. Studies of individual …