Mass spectrometry-based proteomics empowers deep profiling of proteome and protein
posttranslational modifications (PTMs) in Alzheimer's disease (AD). Here we review the …

The development of new ion-activation/dissociation methods continues to be one of the most
active areas of mass spectrometry owing to the broad applications of tandem mass …

Electrospray ionization mass spectrometry (ESI-MS) is nowadays one of the cornerstones of
biomolecular mass spectrometry and proteomics. Advances in sample preparation and …

Charge detection mass spectrometry (CDMS) is a single-particle technique where the
masses of individual ions are determined from simultaneous measurement of their mass-to …

We demonstrate single-particle charge detection mass spectrometry on an Orbitrap for the
analysis of megadalton biomolecular assemblies. We establish that the signal amplitudes of …

Highlights•Collision induced unfolding (CIU) uses gas phase activation to probe
biomolecules.•CIU can detect subtle changes in protein structure, stability or …

Native mass spectrometry (nMS) is evolving into a workhorse for structural biology. The
plethora of online and offline preparation, separation, and purification methods as well as …

Orbitrap-based charge detection mass spectrometry utilizes single-molecule sensitivity to
enable mass analysis of even highly heterogeneous, high-mass macromolecular …

Ammonium acetate (NH4Ac) is a widely used solvent additive in native electrospray
ionization (ESI) mass spectrometry. NH4Ac can undergo proton transfer to form ammonia …

Phosphorylation of proteins is a ubiquitous mechanism of regulating their function,
localization, or activity. Protein kinases, enzymes that use ATP to phosphorylate protein …