Roughly one quarter of all genes code for integral membrane proteins that are inserted into
the plasma membrane of prokaryotes or the endoplasmic reticulum membrane of …

The general secretory (Sec) pathway comprises an essential, ubiquitous and universal
export machinery for most proteins that integrate into, or translocate through, the plasma …

The ubiquitous heat shock protein 70 (HSP70) family consists of ATP-dependent molecular
chaperones, which perform numerous cellular functions that affect almost all aspects of the …

Chemical shift perturbation (CSP, chemical shift mapping or complexation-induced changes
in chemical shift, CIS) follows changes in the chemical shifts of a protein when a ligand is …

A major goal in structural biology is to unravel how molecular machines function in detail. To
that end, solution-state NMR spectroscopy is ideally suited as it is able to study biological …

Introduction Molecular chaperones prevent aggregation and misfolding of proteins in the
cellular environment and are thus central to maintaining protein homeostasis. Molecular …

Most proteins are secreted from bacteria by the interaction of the cytoplasmic SecA ATPase
with a membrane channel, formed by the heterotrimeric SecY complex. Here we report the …

Membrane proteins (MPs) play essential roles in numerous cellular processes. Because
around 70% of the currently marketed drugs target MPs, a detailed understanding of their …

HSP-100 protein machines, such as ClpB, play an essential role in reactivating protein
aggregates that can otherwise be lethal to cells. Although the players involved are known …

The allosteric mechanism of Hsp70 molecular chaperones enables ATP binding to the N-
terminal nucleotide-binding domain (NBD) to alter substrate affinity to the C-terminal …