Efficient genome-editing tools to engineer the recalcitrant non-model industrial microorganism Zymomonas mobilis

G Binan, W Yalun, W Xinyan, Y Yongfu, Z Peng… - Trends in …, 2024 - cell.com
Current biotechnology relies on a few well-studied model organisms, such as Escherichia
coli and Saccharomyces cerevisiae, for which abundant information and efficient toolkits are …

CRISPR-Cas-based engineering of probiotics

L Liu, SE Helal, N Peng - BioDesign Research, 2023 - spj.science.org
Probiotics are the treasure of the microbiology fields. They have been widely used in the
food industry, clinical treatment, and other fields. The equivocal health-promoting effects and …

Systematic analysis of metabolic bottlenecks in the methylerythritol 4-phosphate (MEP) pathway of Zymomonas mobilis

DB Khana, M Tatli, J Rivera Vazquez… - …, 2023 - Am Soc Microbiol
Zymomonas mobilis is an industrially relevant aerotolerant anaerobic bacterium that can
convert up to 96% of consumed glucose to ethanol. This highly catabolic metabolism could …

Towards using bacterial microcompartments as a platform for spatial metabolic engineering in the industrially important and metabolically versatile Zymomonas …

L Doron, D Raval, CA Kerfeld - Frontiers in Bioengineering and …, 2024 - frontiersin.org
Advances in synthetic biology have enabled the incorporation of novel biochemical
pathways for the production of high-value products into industrially important bacterial hosts …

Elimination of editing plasmid mediated by theophylline riboswitch in Zymomonas mobilis

Y Huang, M Chen, G Hu, B Wu, M He - Applied Microbiology and …, 2023 - Springer
Zymomonas mobilis is regarded as a potential chassis for the production of platform
chemicals. Genome editing using the CRISPR-Cas system could meet the need for gene …

Unleashing the potential: type I CRISPR-Cas systems in actinomycetes for genome editing

S Wang, X Zeng, Y Jiang, W Wang, L Bai, Y Lu… - Natural Product …, 2024 - pubs.rsc.org
Covering: up to the end of 2023Type I CRISPR-Cas systems are widely distributed, found in
over 40% of bacteria and 80% of archaea. Among genome-sequenced actinomycetes …

Development of a counterselectable system for rapid and efficient CRISPR-based genome engineering in Zymomonas mobilis

Y Zheng, H Fu, J Chen, J Li, Y Bian, P Hu, L Lei… - Microbial Cell …, 2023 - Springer
Background Zymomonas mobilis is an important industrial bacterium ideal for biorefinery
and synthetic biology studies. High-throughput CRISPR-based genome editing technologies …

Improving Fructooligosaccharide Production via sacC Gene Deletion in Zymomonas mobilis: A Novel Approach for Enhanced Prebiotic Production

A Braga, AB Maia, D Gomes, JL Rodrigues… - Food and Bioprocess …, 2024 - Springer
Fructooligosaccharides (FOS) are promising prebiotics in the relevant and increasing market
of functional food. Industrially, these compounds are produced from sucrose by the action of …

Identification and Characterization of Genes Related to Ampicillin Antibiotic Resistance in Zymomonas mobilis

B Geng, X Huang, Y Wu, Q He, S Yang - Antibiotics, 2022 - mdpi.com
Antibiotics can inhibit or kill microorganisms, while microorganisms have evolved antibiotic
resistance strategies to survive antibiotics. Zymomonas mobilis is an ideal industrial …

Lipid membrane remodeling and metabolic response during isobutanol and ethanol exposure in Zymomonas mobilis

J Rivera Vazquez, E Trujillo, J Williams, F She… - … for Biofuels and …, 2024 - Springer
Background Recent engineering efforts have targeted the ethanologenic bacterium
Zymomonas mobilis for isobutanol production. However, significant hurdles remain due this …