Polyhydroxyalkanoates bioproduction from bench to industry: thirty years of development towards sustainability

PA Diankristanti, YC Lin, YC Yi, IS Ng - Bioresource technology, 2023 - Elsevier
The pursuit of carbon neutrality goals has sparked considerable interest in expanding
bioplastics production from microbial cell factories. One prominent class of bioplastics …

Challenges and opportunities of bioprocessing 5-aminolevulinic acid using genetic and metabolic engineering: a critical review

YC Yi, IT Shih, TH Yu, YJ Lee, IS Ng - Bioresources and Bioprocessing, 2021 - Springer
Aminolevulinic acid (5-ALA), a non-proteinogenic five-carbon amino acid, has received
intensive attentions in medicine due to its approval by the US Food and Drug Administration …

Recent Advances in Engineering Heterotrophic Microorganisms for Reinforcing CO2 Fixation Based on Calvin–Benson–Bassham Cycle

J Feng, D Ma, S Gao, Y Liao, J Feng, S Xu… - ACS Sustainable …, 2023 - ACS Publications
Global climate change caused by greenhouse gas emission is the fundamental challenge
facing mankind. Biological carbon dioxide fixation has recently attracted much attention. The …

Enhanced recombinant carbonic anhydrase in T7RNAP-equipped Escherichia coli W3110 for carbon capture storage and utilization (CCSU)

WW Ting, JY Yu, YC Lin, IS Ng - Bioresource technology, 2022 - Elsevier
Sulfurihydrogenibium yellowstonense carbonic anhydrase (SyCA) is a well-known
thermophilic CA for carbon mineralization. To broaden the applications of SyCA, the activity …

Adaptive laboratory evolution and metabolic regulation of genetic Escherichia coli W3110 toward low-carbon footprint production of 5-aminolevulinic acid

WW Ting, IS Ng - Journal of the Taiwan Institute of Chemical Engineers, 2022 - Elsevier
Abstract Background The 5-aminolevulinic acid (5-ALA) is a prodrug that has been
approved by FDA for photodynamic therapy of cancer. 5-ALA is intracellularly metabolized to …

Non-native Pathway Engineering with CRISPRi for Carbon Dioxide Assimilation and Valued 5-Aminolevulinic Acid Synthesis in Escherichia coli Nissle

SSW Effendi, IS Ng - ACS Synthetic Biology, 2024 - ACS Publications
Carbon dioxide emission and acidification during chemical biosynthesis are critical
challenges toward microbial cell factories' sustainability and efficiency. Due to its acidophilic …

Applications of synthetic biotechnology on carbon neutrality research: a review on electrically driven microbial and enzyme engineering

X Zhuang, Y Zhang, AF Xiao, A Zhang… - … in Bioengineering and …, 2022 - frontiersin.org
With the advancement of science, technology, and productivity, the rapid development of
industrial production, transportation, and the exploitation of fossil fuels has gradually led to …

Low-Carbon-Footprint Production of High-End 5-Aminolevulinic Acid via Integrative Strain Engineering and RuBisCo-Equipped Escherichia coli

YC Yi, C Xue, IS Ng - ACS Sustainable Chemistry & Engineering, 2021 - ACS Publications
5-Aminolevulinic acid (ALA) is an imperative compound that has been widely applied in the
agricultural and medical fields. The ALA synthesis in Escherichia coli via the C4 pathway …

Effective whole cell biotransformation of arginine to a four-carbon diamine putrescine using engineered Escherichia coli

SC Yang, WW Ting, IS Ng - Biochemical Engineering Journal, 2022 - Elsevier
The rising awareness of environmental protection has triggered bio-based materials to
replace the traditional petrochemical plastics. Putrescine as 1, 4-diaminobutane is an …

From Rags to Riches: Exploiting the Calvin‐Benson‐Bassham Cycle for Biomanufacturing

F Federici, E Orsi, PI Nikel - ChemCatChem, 2023 - Wiley Online Library
Industrial chemical production largely relies on fossil fuels, resulting in the unavoidable
release of carbon dioxide (CO2) into the atmosphere. The concept of a circular carbon …