The formation of new blood vessels, or angiogenesis, is a complex process that plays
important roles in growth and development, tissue and organ regeneration, as well as …

Zebrafish has many advantages as a model of human pediatric research. Given the physical
and ethical problems with performing experiments on human patients, biomedical research …

Three-dimensional cell culture models have either relied on the self-organizing properties of
mammalian cells 1, 2, 3, 4, 5, 6 or used bioengineered constructs to arrange cells in an …

Vessel sprouting by migrating tip and proliferating stalk endothelial cells (ECs) is controlled
by genetic signals (such as Notch), but it is unknown whether metabolism also regulates this …

Cell-cell junctions respond to mechanical forces by changing their organization and
function. To gain insight into the mechanochemical basis underlying junction …

CRISPR-Cas9 technology would be enhanced by the ability to inhibit Cas9 function
spatially, temporally, or conditionally. Previously, we discovered small proteins encoded by …

Transgenesis is an important tool for assessing gene function. In zebrafish, transgenesis has
suffered from three problems: the labor of building complex expression constructs using …

INTRODUCTION The “cancerized field” concept posits that cells in a given tissue sharing an
oncogenic mutation are cancer-prone, yet only discreet clones within the field initiate tumors …

Molecular genetics approaches in zebrafish research are hampered by the lack of a
ubiquitous transgene driver element that is active at all developmental stages. Here, we …

Embryo development requires biochemical signalling to generate patterns of cell fates and
active mechanical forces to drive tissue shape changes. However, how these processes are …