Integrating biological redesign: where synthetic biology came from and where it needs to go
Synthetic biology seeks to extend approaches from engineering and computation to
redesign of biology, with goals such as generating new chemicals, improving human health …
redesign of biology, with goals such as generating new chemicals, improving human health …
In vivo co-localization of enzymes on RNA scaffolds increases metabolic production in a geometrically dependent manner
Co-localization of biochemical processes plays a key role in the directional control of
metabolic fluxes toward specific products in cells. Here, we employ in vivo scaffolds made of …
metabolic fluxes toward specific products in cells. Here, we employ in vivo scaffolds made of …
Construction of a linker library with widely controllable flexibility for fusion protein design
Flexibility or rigidity of the linker between two fused proteins is an important parameter that
affects the function of fusion proteins. In this study, we constructed a linker library with five …
affects the function of fusion proteins. In this study, we constructed a linker library with five …
Design and characterization of a nanopore-coupled polymerase for single-molecule DNA sequencing by synthesis on an electrode array
Scalable, high-throughput DNA sequencing is a prerequisite for precision medicine and
biomedical research. Recently, we presented a nanopore-based sequencing-by-synthesis …
biomedical research. Recently, we presented a nanopore-based sequencing-by-synthesis …
Engineering bacterial translation initiation—Do we have all the tools we need?
Background Reliable tools that allow precise and predictable control over gene expression
are critical for the success of nearly all bioengineering applications. Translation initiation is …
are critical for the success of nearly all bioengineering applications. Translation initiation is …
Activation of acid-sensing ion channels by localized proton transient reveals their role in proton signaling
Extracellular transients of pH alterations likely mediate signal transduction in the nervous
system. Neuronal acid-sensing ion channels (ASICs) act as sensors for extracellular …
system. Neuronal acid-sensing ion channels (ASICs) act as sensors for extracellular …
Introduction to focus issue: quantitative approaches to genetic networks
All cells of living organisms contain similar genetic instructions encoded in the organism's
DNA. In any particular cell, the control of the expression of each different gene is regulated …
DNA. In any particular cell, the control of the expression of each different gene is regulated …
Bioinspired Design of Artificial Signaling Systems
JC Way, DR Burrill, PA Silver - Biochemistry, 2022 - ACS Publications
Natural systems use weak interactions and avidity effects to give biological systems high
specificity and signal-to-noise ratios. Here we describe design principles for engineering …
specificity and signal-to-noise ratios. Here we describe design principles for engineering …
[HTML][HTML] Designing cell-targeted therapeutic proteins reveals the interplay between domain connectivity and cell binding
A Robinson-Mosher, JH Chen, J Way, PA Silver - Biophysical Journal, 2014 - cell.com
The therapeutic efficacy of cytokines is often hampered by severe side effects due to their
undesired binding to healthy cells. One strategy for overcoming this obstacle is to tether …
undesired binding to healthy cells. One strategy for overcoming this obstacle is to tether …
[图书][B] rpsA and ribosomal protein S1: investigating a non-canonical translation initiation element
JRJ Vigar - 2019 - search.proquest.com
Translation initiation rates are fine-tuned by altering interactions between the ribosome and
the translation initiation region of an mRNA. In bacteria varying levels of RNA structure …
the translation initiation region of an mRNA. In bacteria varying levels of RNA structure …