[HTML][HTML] Nanopore sequencing technology, bioinformatics and applications

Y Wang, Y Zhao, A Bollas, Y Wang, KF Au - Nature biotechnology, 2021 - nature.com
Rapid advances in nanopore technologies for sequencing single long DNA and RNA
molecules have led to substantial improvements in accuracy, read length and throughput …

Long-read human genome sequencing and its applications

GA Logsdon, MR Vollger, EE Eichler - Nature Reviews Genetics, 2020 - nature.com
Over the past decade, long-read, single-molecule DNA sequencing technologies have
emerged as powerful players in genomics. With the ability to generate reads tens to …

The architecture of SARS-CoV-2 transcriptome

D Kim, JY Lee, JS Yang, JW Kim, VN Kim, H Chang - Cell, 2020 - cell.com
SARS-CoV-2 is a betacoronavirus responsible for the COVID-19 pandemic. Although the
SARS-CoV-2 genome was reported recently, its transcriptomic architecture is unknown …

Identification of differential RNA modifications from nanopore direct RNA sequencing with xPore

PN Pratanwanich, F Yao, Y Chen, CWQ Koh… - Nature …, 2021 - nature.com
RNA modifications, such as N 6-methyladenosine (m6A), modulate functions of cellular RNA
species. However, quantifying differences in RNA modifications has been challenging. Here …

Opportunities and challenges in long-read sequencing data analysis

SL Amarasinghe, S Su, X Dong, L Zappia, ME Ritchie… - Genome biology, 2020 - Springer
Long-read technologies are overcoming early limitations in accuracy and throughput,
broadening their application domains in genomics. Dedicated analysis tools that take into …

Systematic comparison of tools used for m6A mapping from nanopore direct RNA sequencing

ZD Zhong, YY Xie, HX Chen, YL Lan, XH Liu… - Nature …, 2023 - nature.com
Abstract N6-methyladenosine (m6A) has been increasingly recognized as a new and
important regulator of gene expression. To date, transcriptome-wide m6A detection primarily …

Quantitative profiling of pseudouridylation dynamics in native RNAs with nanopore sequencing

O Begik, MC Lucas, LP Pryszcz, JM Ramirez… - Nature …, 2021 - nature.com
Nanopore RNA sequencing shows promise as a method for discriminating and identifying
different RNA modifications in native RNA. Expanding on the ability of nanopore sequencing …

Detection of m6A from direct RNA sequencing using a multiple instance learning framework

C Hendra, PN Pratanwanich, YK Wan, WSS Goh… - Nature …, 2022 - nature.com
RNA modifications such as m6A methylation form an additional layer of complexity in the
transcriptome. Nanopore direct RNA sequencing can capture this information in the raw …

From squiggle to basepair: computational approaches for improving nanopore sequencing read accuracy

FJ Rang, WP Kloosterman, J de Ridder - Genome biology, 2018 - Springer
Nanopore sequencing is a rapidly maturing technology delivering long reads in real time on
a portable instrument at low cost. Not surprisingly, the community has rapidly taken up this …

Genomics in the long-read sequencing era

EL van Dijk, D Naquin, K Gorrichon, Y Jaszczyszyn… - Trends in Genetics, 2023 - cell.com
Long-read sequencing (LRS) technologies have provided extremely powerful tools to
explore genomes. While in the early years these methods suffered technical limitations, they …