Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

The redox states of the NAD and NADP pyridine nucleotide pools play critical roles in
defining the activity of energy producing pathways, in driving oxidative stress and in …

There is a growing interest in developing microphysiological systems that can be used to
model both normal and pathological human organs in vitro. This “organs-on-chips” …

NAD is a key determinant of cellular energy metabolism. In contrast, its phosphorylated form,
NADP, plays a central role in biosynthetic pathways and antioxidant defence. The reduced …

Fluorescence lifetime imaging (FLIM) uses the fact that the fluorescence lifetime of a
fluorophore depends on its molecular environment but not on its concentration. Molecular …

Purpose: Cancer treatment is limited by inaccurate predictors of patient-specific therapeutic
response. Therefore, some patients are exposed to unnecessary side effects and delays in …

Cells are the basic units of all living matter which harness the flow of energy to drive the
processes of life. While the biochemical networks involved in energy transduction are well …

Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

Metabolic imaging of the relative amounts of reduced NADH and FAD and the
microenvironment of these metabolic electron carriers can be used to noninvasively monitor …

The interaction of cells within their microenvironmental niche is fundamental to cell
migration, positioning, growth and differentiation in order to form and maintain complex …