Formate dehydrogenases (FDHs) catalyze the oxidation of formate to CO2 while reducing
NAD (P)+ to NAD (P) H and are classified into two main classes: metal‐dependent (Mo‐or W …

In this work, the activity of horseradish peroxidase (HRP) in betaine-based natural deep
eutectic systems (NADESs) was measured and studied by molecular simulations. Focus was …

Engineering of reaction media is an exciting alternative for modulating kinetic properties of
biocatalytic reactions. We addressed the combined effect of an aqueous two-phase system …

In recent years, oncolytic viruses (OVs) have emerged as an effective means of treating
cancer. OVs have multiple oncotherapeutic functions including specifically infecting and …

Specifically designed co-solvent mixtures are an efficient way to enhance the kinetics of
enzyme-catalyzed reactions without compromising enzyme stability; among them, several …

The study of chemical reactions in multiple liquid phase systems is becoming more and
more relevant in industry and academia. The ability to predict combined chemical and phase …

The ability of bovine serum albumin (BSA) to form condensates in crowded environments
has been discovered only recently. Effects of this condensed state on the secondary …

Abstract α-amylases are industrially important enzymes which are used in different starch-
based industries. They are adapted to different environmental conditions like extremes of …

Optimizing the liquid reaction phase holds significant potential for enhancing the efficiency
of biocatalytic processes since it determines reaction equilibrium and kinetics. This study …

The thesis herein presented reflects the work developed from 2020 to 2024 in the
Biocatalysis & Bioenergy (B&B) group under the supervision of Dr. Alexandre Babo de …