Advances in high-speed atomic force microscopy (HS-AFM) reveal dynamics of transmembrane channels and transporters
GR Heath, S Scheuring - Current opinion in structural biology, 2019 - Elsevier
Highlights•HS-AFM detects conformational changes of unlabeled membrane proteins.•In situ
responses to pH, ligands, temperature and light can be visualized.•Developments in …
responses to pH, ligands, temperature and light can be visualized.•Developments in …
Current problems and future avenues in proteoliposome research
AM Amati, S Graf, S Deutschmann… - Biochemical Society …, 2020 - portlandpress.com
Membrane proteins (MPs) are the gatekeepers between different biological compartments
separated by lipid bilayers. Being receptors, channels, transporters, or primary pumps, they …
separated by lipid bilayers. Being receptors, channels, transporters, or primary pumps, they …
Voltage sensors of a Na+ channel dissociate from the pore domain and form inter-channel dimers in the resting state
Understanding voltage-gated sodium (Nav) channels is significant since they generate
action potential. Nav channels consist of a pore domain (PD) and a voltage sensor domain …
action potential. Nav channels consist of a pore domain (PD) and a voltage sensor domain …
Millisecond dynamic of SARS‐CoV‐2 spike and its interaction with ACE2 receptor and small extracellular vesicles
Abstract SARS‐CoV‐2 spike protein (S) binds to human angiotensin‐converting enzyme 2
(hACE2), allowing virus to dock on cell membrane follow by viral entry. Here, we use high …
(hACE2), allowing virus to dock on cell membrane follow by viral entry. Here, we use high …
Fabrication and filtration performance of aquaporin biomimetic membranes for water treatment
A Beratto-Ramos, J Dagnino-Leone… - Separation & …, 2022 - Taylor & Francis
Desalination is considered one of the strongest alternatives to face global water scarcity.
Since conventional reverse osmosis is an energy-intensive process, Aquaporin biomimetic …
Since conventional reverse osmosis is an energy-intensive process, Aquaporin biomimetic …
Cardiolipin binding enhances KcsA channel gating via both its specific and dianion-monoanion interchangeable sites
KcsA is a potassium channel with a plethora of structural and functional information, but its
activity in the KcsA-producing actinomycete membranes remains elusive. To determine lipid …
activity in the KcsA-producing actinomycete membranes remains elusive. To determine lipid …
Single-unit imaging of membrane protein-embedded nanodiscs from two oriented sides by high-speed atomic force microscopy
Membrane proteins play important roles in various cellular functions. To analyze membrane
proteins, nanodisc technology using membrane scaffold proteins allows single membrane …
proteins, nanodisc technology using membrane scaffold proteins allows single membrane …
High-speed AFM reveals accelerated binding of agitoxin-2 to a K+ channel by induced fit
Agitoxin-2 (AgTx2) from scorpion venom is a potent blocker of K+ channels. The docking
model has been elucidated, but it remains unclear whether binding dynamics are described …
model has been elucidated, but it remains unclear whether binding dynamics are described …
Queueing arrival and release mechanism for K+ permeation through a potassium channel
T Sumikama, S Oiki - The Journal of Physiological Sciences, 2019 - Springer
The mechanism underlying ion permeation through potassium channels still remains
controversial. K+ ions permeate across a narrow selectivity filter (SF) in a single file …
controversial. K+ ions permeate across a narrow selectivity filter (SF) in a single file …
Channel formation and membrane deformation via sterol-aided polymorphism of amphidinol 3
Abstract Amphidinol 3 (AM3) is an anti-fungal polyene extracted from a marine
dinoflagellate. Here, we examined the ion channel activity and membrane-embedded …
dinoflagellate. Here, we examined the ion channel activity and membrane-embedded …