Crosslinking mass spectrometry (crosslinking-MS) is a versatile tool providing structural
insights into protein conformation and protein-protein interactions. Its medium-resolution …

While AlphaFold2 can predict accurate protein structures from the primary sequence,
challenges remain for proteins that undergo conformational changes or for which few …

Protein-protein interactions govern most cellular pathways and processes, and multiple
technologies have emerged to systematically map them. Assessing the error of interaction …

Synaptic vesicles are storage organelles for neurotransmitters. They pass through a
trafficking cycle and fuse with the pre-synaptic membrane when an action potential arrives at …

The field of structural biology is increasingly focusing on studying proteins in situ, ie, in their
greater biological context. Cross-linking mass spectrometry (CLMS) is contributing to this …

Crosslinking mass spectrometry has developed into a robust technique that is increasingly
used to investigate the interactomes of organelles and cells. However, the incomplete and …

Protein synthesis begins with the formation of a ribosome-messenger RNA (mRNA)
complex. In bacteria, the small ribosomal subunit (30 S) is recruited to many mRNAs through …

Proteome-wide crosslinking mass spectrometry studies have coincided with the advent of
mass spectrometry (MS)-cleavable crosslinkers that can reveal the individual masses of the …

Membrane proteins are key players of the cell. Their structure and the interactions they form
with their lipid environment are required to understand their function. Here we explore …

Ion-mobility spectrometry shows great promise to tackle analytically challenging research
questions by adding another separation dimension to liquid chromatography–mass …