Process engineering of human pluripotent stem cells for clinical application

M Serra, C Brito, C Correia, PM Alves - Trends in biotechnology, 2012 - cell.com
Human pluripotent stem cells (hPSCs), including embryonic and induced pluripotent stem
cells, constitute an extremely attractive tool for cell therapy. However, flexible platforms for …

Concise review: the evolution of human pluripotent stem cell culture: from feeder cells to synthetic coatings

LG Villa-Diaz, AM Ross, J Lahann, PH Krebsbach - Stem cells, 2013 - academic.oup.com
Current practices to maintain human pluripotent stem cells (hPSCs), which include induced
pluripotent stem cells and embryonic stem cells, in an undifferentiated state typically depend …

Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage.

K Amps, PW Andrews, G Anyfantis… - Nature …, 2011 - search.ebscohost.com
Abstract The International Stem Cell Initiative analyzed 125 human embryonic stem (ES) cell
lines and 11 induced pluripotent stem (iPS) cell lines, from 38 laboratories worldwide, for …

Role of E-cadherin and other cell adhesion molecules in survival and differentiation of human pluripotent stem cells

L Li, SAL Bennett, L Wang - Cell adhesion & migration, 2012 - Taylor & Francis
The survival, proliferation, self-renewal and differentiation of human pluripotent stem cells
(hPSCs, including human embryonic stem cells and human induced pluripotent stem cells) …

Highly efficient genome editing via CRISPR–Cas9 in human pluripotent stem cells is achieved by transient BCL-XL overexpression

XL Li, GH Li, J Fu, YW Fu, L Zhang, W Chen… - Nucleic acids …, 2018 - academic.oup.com
Genome editing of human induced pluripotent stem cells (iPSCs) is instrumental for
functional genomics, disease modeling, and regenerative medicine. However, low editing …

Genomic instability in pluripotent stem cells: implications for clinical applications

SE Peterson, JF Loring - Journal of Biological Chemistry, 2014 - ASBMB
Human pluripotent stem cells (hPSCs) are known to acquire genomic changes as they
proliferate and differentiate. Despite concerns that these changes will compromise the safety …

Human embryonic stem cells have constitutively active Bax at the Golgi and are primed to undergo rapid apoptosis

R Dumitru, V Gama, BM Fagan, JJ Bower, V Swahari… - Molecular cell, 2012 - cell.com
Human embryonic stem (hES) cells activate a rapid apoptotic response after DNA damage
but the underlying mechanisms are unknown. A critical mediator of apoptosis is Bax, which …

iPS-cell technology and the problem of genetic instability—Can it ever be safe for clinical use?

SW Attwood, MJ Edel - Journal of clinical medicine, 2019 - mdpi.com
The use of induced Pluripotent Stem Cells (iPSC) as a source of autologous tissues shows
great promise in regenerative medicine. Nevertheless, several major challenges remain to …

Gain of 20q11. 21 in human embryonic stem cells improves cell survival by increased expression of Bcl-xL

HT Nguyen, M Geens, A Mertzanidou… - Molecular human …, 2014 - academic.oup.com
Abstract Gain of 20q11. 21 is a chromosomal abnormality that is recurrently found in human
pluripotent stem cells and cancers, strongly suggesting that this mutation confers a …

Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage

Nature biotechnology, 2011 - nature.com
Abstract The International Stem Cell Initiative analyzed 125 human embryonic stem (ES) cell
lines and 11 induced pluripotent stem (iPS) cell lines, from 38 laboratories worldwide, for …