Oxidative stress is generally considered as the consequence of an imbalance between pro-
and antioxidants species, which often results into indiscriminate and global damage at the …

Recent advances in enzyme engineering enable dramatic improvements in catalytic
efficiency and/or selectivity, as well as de novo engineering of enzymes to catalyze reactions …

Novel approaches are needed to combat antibiotic resistance. Here, we describe a
computational–experimental framework for the discovery of novel cryptic antimicrobial …

Hydroxytyrosol (HT), which is a polyphenol with a high antioxidant power and many
associated health benefits, has been found in wines. Wine yeasts are capable of producing …

Toluene o-xylene monooxygenase (ToMO) was found to oxidize chlorobenzene to form 2-
chlorophenol (2-CP, 4%), 3-CP (12%), and 4-CP (84%) with a total product formation rate of …

Bioapplications of 2D materials embrace demanding features in terms of environmental
impact, toxicity and biocompatibility. Here we report on the use of a rationally modified …

Enzyme promiscuity plays an important role in developing biosynthetic pathways for novel
target products. Phenol hydroxylase (PH) from Pseudomonas stutzeri OX1 is capable of …

Crystal structures of toluene 4-monooxygenase hydroxylase in complex with reaction
products and effector protein reveal active site interactions leading to regiospecificity …

An engineered Escherichia coli was constructed by co-expressing L-amino acid deaminase,
α-keto acid decarboxylase, alcohol dehydrogenase, and glucose dehydrogenase through …

Oxygenases are ubiquitous enzymes that catalyze the introduction of one or two oxygen
atoms to unreactive chemical compounds. They require reduction equivalents from NADH or …