Productive transfection and gene transfer require not simply the entry of DNA into cells and
subsequent transcription from an appropriate promoter, but also a number of intracellular …

Lipoplexes and polyplexes, electrostatic complexes between a plasmid DNA and cationic
lipids or polymers are chemical systems that are developed for gene delivery. Considerable …

DNA in cells exhibits a supercoiled state in which the double helix is additionally twisted to
form extended intertwined loops called plectonemes. Although supercoiling is vital to many …

One of the limiting steps in the efficiency of nonviral gene delivery is transport of genetic
material across the nuclear membrane. Trafficking of nuclear proteins from the cytoplasm …

DNA supercoiling crucially affects cellular processes such as DNA replication, gene
expression, and chromatin organization. However, mechanistic understanding of DNA …

Publisher Summary This chapter focuses on the delivery of DNA by various physical
methods such as electroporation (EP), sonoporation, microinjection, particle bombardment …

Background There has been considerable interest in researching the regulatory
mechanisms that control transgene expression. In particular, there is impetus to investigate …

This short review deals with some properties of nuclear sugar-binding proteins also called
nuclear lectins, the sugar-dependent nuclear import of neoglycoproteins and the attempts of …

Live-cell imaging has provided the life sciences with insights into the cell biology and
dynamics. Fluorescent labeling of target molecules proves to be indispensable in this …

Cationic membrane disruptive peptides such as melittin would appear to have attributes
necessary for DNA delivery: DNA binding via electrostatic interactions and membrane lysis …