Crystal structure of a family 16 endoglucanase from the hyperthermophile Pyrococcus furiosus– structural basis of substrate recognition

A Ilari, A Fiorillo, S Angelaccio, R Florio… - The FEBS …, 2009 - Wiley Online Library
Bacterial and archaeal endo‐β‐1, 3‐glucanases that belong to glycoside hydrolase family
16 share a β‐jelly‐roll fold, but differ significantly in sequence and in substrate specificity …

Purification, cDNA cloning and homology modeling of endo-1, 3-β-D-glucanase from scallop Mizuhopecten yessoensis

SN Kovalchuk, EV Sundukova, MI Kusaykin… - … and Physiology Part B …, 2006 - Elsevier
The retaining endo-1, 3-β-d-glucanase (LV) with molecular mass of 36 kDa was purified to
homogeneity from the crystalline styles of scallop Mizuhopecten yessoensis. The purified …

Thermal stabilization of an endoglucanase by cyclization

JFT Van Lieshout, ONP Gutiérrez, W Vroom… - Applied biochemistry …, 2012 - Springer
An intein-driven protein splicing approach allowed for the covalent linkage between the N-
and C-termini of a polypeptide chain to create circular variants of the endo-β-1, 3-1, 4 …

Tertiary structure in 7.9 m guanidinium chloride − the role of Glu53 and Asp287 in Pyrococcus furiosus endo‐β‐1,3‐glucanase

R Chiaraluce, R Florio, S Angelaccio… - The FEBS …, 2007 - Wiley Online Library
The thermodynamic stability of family 16 endo‐β‐1, 3‐glucanase (EC 3.2. 1.39) from the
hyperthermophilic archaeon Pyrococcus furiosus is decreased upon single (D287A, E53A) …

[PDF][PDF] Stabilization of an Endo-β-1, 3-1, 4-Glucanase by Cyclization

JFT Van Lieshout, ON Pérez Gutiérrez, W Vroom… - academia.edu
A novel protein-engineering approach has recently been developed on the basis of intein-
driven protein splicing. A modification of this process allows for the generation of a covalent …

NMR characterization of a 264-residue hyperthermostable endo-β-1, 3-glucanase

JH Ippel, S Koutsopoulos, SM Nabuurs… - Biochemical and …, 2010 - Elsevier
Insight into the hyperthermostable endo-β-1, 3-glucanase pfLamA from Pyrococcus furiosus
is obtained by using NMR spectroscopy. pfLamA functions optimally at 104° C and recently …

[PDF][PDF] Федеральное государственное бюджетное учреждение науки Тихоокеанский Институт Географии Дальневосточного отделения Российской Академии …

ДС Агеев - 2014 - igc.irk.ru
Под аукционом в электронной форме (далее-электронный аукцион) понимается
аукцион, при котором информация о закупке сообщается заказчиком неограниченному …

Stabilization of an Endo-ß-1, 3-1, 4-Glucanase by Cyclization

JFT Van Lieshout, ON Perez-Gutierrez… - … and engineering of …, 2007 - search.proquest.com
A novel protein-engineering approach has recently been developed on the basis of intein-
driven protein splicing. A modification of this process allows for the generation of a covalent …